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PMID: 3138110 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Segmental flexibility and complement fixation of genetically engineered chimeric human, rabbit and mouse antibodies.

The EMBO journal ·Vol. 7 ·No. 7 ·1988-07-00 ·Pages 1989-94

Dangl JL, Wensel TG, Morrison SL, Stryer L, Herzenberg LA, Oi VT

Abstract

We generated a family of chimeric immunoglobulin G (IgG) molecules having identical antigen-combining sites for the dansyl (DNS) hapten, in conjunction with nine heavy chain constant (CH) regions. This family of antibody molecules allows comparison of CH dependent properties independent of possible variable region contributions to IgG function. The segmental flexibility and complement fixation activity were measured of six genetically engineered molecules (the four human IgG isotypes, mouse IgG3 and rabbit IgG) and the remaining three mouse IgG isotypes, (IgG1, IgG2a and IgG2b), isolated previously by somatic cell genetic techniques. These properties of antibody molecules each correlate with the length of the immunoglobulin hinge region which separate the first and second CH (CH1 and CH2) domains. These results attribute a structural basis for two critical properties of antibody molecules.

MeSH Terms
Amino Acid Sequence Animals Chimera Complement Fixation Tests Humans Immunoglobulin Constant Regions/genetics Immunoglobulin G/genetics,immunology Immunoglobulin Heavy Chains/genetics Mice Molecular Sequence Data Rabbits Recombinant Proteins/immunology Species Specificity
Chemicals
Immunoglobulin Constant Regions Immunoglobulin G Immunoglobulin Heavy Chains Recombinant Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Dangl J L
Department of Genetics, Stanford University, CA 94305.
Wensel T G
Morrison S L
Stryer L
Herzenberg L A
Oi V T
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-07-00
Pages
1989-94
Language
English
Region
England
NLM ID
8208664
PMCID
PMC454472
Subset
IM
Grants
NIGMS NIH HHS · 2-T32-GM-07790 · United States
NCI NIH HHS · CA-04681 · United States
NICHD NIH HHS · HD-01287 · United States
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PubMed source PMC Full Text View journal details
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