Home LiteratureArticle Details
PMID: 3137351 Published · ppublish English Journal Article

The complete nucleotide sequence of the small-subunit ribosomal RNA coding region for the cycad Zamia pumila: phylogenetic implications.

Journal of molecular evolution ·Vol. 27 ·No. 2 ·1988-00-00 ·Pages 133-41

Nairn CJ, Ferl RJ

Abstract

The DNA sequence of the small-subunit ribosomal RNA coding region for the cycad Zamia pumila L. was determined. The Zamia small-subunit rRNA was found to be 1813 nucleotides in length and approximately 92% identical to published angiosperm small-subunit rRNA sequences. Conserved regions interspersed with variable regions are observed corresponding to those found in other eukaryotic small-subunit sequences. Using representatives from protist, fungal, plant, and animal groups, a distance matrix was constructed of average nucleotide substitution rates for pairs of organisms. Phylogenetic trees were inferred from similarities between sequences. The sequence of Zamia represents the earliest divergence from the higher plant lineage reported to date for small-subunit rRNA data. Inferred phylogenies also support a monophyletic origin for the angiosperms consistent with studies citing phenotypic characters.

MeSH Terms
Base Sequence Macromolecular Substances Molecular Sequence Data Phylogeny Plants/genetics RNA, Ribosomal/genetics Trees
Chemicals
Macromolecular Substances RNA, Ribosomal
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nairn C J
Department of Botany, University of Florida, Gainesville 32611.
Ferl R J
References (34)
34 references, click to expand
  1. Construction of phylogenetic trees.
    Science. 1967 Jan 20;155(3760):279-84 PMID: 5334057
  2. A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the corn mitochondrial 18 S rDNA.
    Plasmid. 1985 Jan;13(1):31-40 PMID: 3991809
  3. Dinoflagellate 17S rRNA sequence inferred from the gene sequence: Evolutionary implications.
    Proc Natl Acad Sci U S A. 1986 Nov;83(22):8644-8 PMID: 16578795
  4. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  5. Lambda replacement vectors carrying polylinker sequences.
    J Mol Biol. 1983 Nov 15;170(4):827-42 PMID: 6315951
  6. The complete nucleotide sequence of the rat 18S ribosomal RNA gene and comparison with the respective yeast and frog genes.
    Nucleic Acids Res. 1983 Jul 25;11(14):4879-90 PMID: 6308563
  7. A comprehensive sequence analysis program for the IBM personal computer.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):581-99 PMID: 6546431
  8. Nucleotide sequence of Xenopus laevis 18S ribosomal RNA inferred from gene sequence.
    Nature. 1981 May 21;291(5812):205-8 PMID: 7015146
  9. Identification of ten additional nucleotides in the primary structure of yeast 18S rRNA.
    Gene. 1986;44(1):143-5 PMID: 3021584
  10. The small-subunit ribosomal RNA gene sequences from the hypotrichous ciliates Oxytricha nova and Stylonychia pustulata.
    Mol Biol Evol. 1985 Sep;2(5):399-410 PMID: 3939705
  11. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  12. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  13. Nucleotide sequence of Dictyostelium discoideum 5.8S ribosomal ribonucleic acid: evolutionary and secondary structural implications.
    Biochemistry. 1982 May 11;21(10):2335-43 PMID: 7093192
  14. Structure and function of ribosomal RNA.
    Biochem J. 1985 Jul 1;229(1):1-17 PMID: 3899100
  15. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.
    Biochemistry. 1977 Oct 18;16(21):4743-51 PMID: 911786
  16. Superoxide dismutase in photosynthetic organisms provides an evolutionary hypothesis.
    Nature. 1975 Oct 23;257(5528):670-2 PMID: 810720
  17. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  18. The interpretation of studies on rapidly labeled ribonucleic Acid in higher plants.
    Plant Physiol. 1973 Feb;51(2):412-4 PMID: 16658343
  19. Evolutionary change in 5S RNA secondary structure and a phylogenic tree of 54 5S RNA species.
    Proc Natl Acad Sci U S A. 1979 Jan;76(1):381-5 PMID: 284354
  20. Evolutionary diversity of eukaryotic small-subunit rRNA genes.
    Proc Natl Acad Sci U S A. 1986 Mar;83(5):1383-7 PMID: 2419907
  21. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  22. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  23. Comparison of the nucleotide sequence of soybean 18S rRNA with the sequences of other small-subunit rRNAs.
    J Mol Evol. 1984-1985;21(3):259-69 PMID: 6100313
  24. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  25. Molecules as documents of evolutionary history.
    J Theor Biol. 1965 Mar;8(2):357-66 PMID: 5876245
  26. Cloning and sequencing of the ribosomal RNA genes in maize: the 17S region.
    DNA. 1984;3(1):31-40 PMID: 6365492
  27. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  28. Phylogenetic relationships among eukaryotic kingdoms inferred from ribosomal RNA sequences.
    J Mol Evol. 1985;22(1):32-8 PMID: 3932662
  29. The complete nucleotide sequence of a rice 17S rRNA gene.
    Nucleic Acids Res. 1984 Jul 11;12(13):5441-8 PMID: 6087286
  30. A practical method for calculating evolutionary trees from sequence data.
    J Theor Biol. 1981 Jul 21;91(2):261-72 PMID: 7300391
  31. Comparative anatomy of 16-S-like ribosomal RNA.
    Prog Nucleic Acid Res Mol Biol. 1985;32:155-216 PMID: 3911275
  32. On the stochastic model for estimation of mutational distance between homologous proteins.
    J Mol Evol. 1972 Dec 29;2(1):87-90 PMID: 4668865
  33. Evolution of green plants as deduced from 5S rRNA sequences.
    Proc Natl Acad Sci U S A. 1985 Feb;82(3):820-3 PMID: 16593540
  34. The molecular evolution of cytochrome c in eukaryotes.
    J Mol Evol. 1976 Jun 23;8(1):13-40 PMID: 181584
Article Info
Journal
Journal of molecular evolution
Abbr.
J Mol Evol
ISSN
0022-2844
DOI
10.1007/BF02138373
Published
1988-00-00
Pages
133-41
Language
English
Region
Germany
NLM ID
0360051
Subset
IM
Databases
GENBANK
M20017
External Links
PubMed source DOI: 10.1007/BF02138373 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2