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PMID: 3120192 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Zinc (II) and the single-stranded DNA binding protein of bacteriophage T4.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 23 ·1987-12-00 ·Pages 8515-9

Gauss P, Krassa KB, McPheeters DS, Nelson MA, Gold L

Abstract

The DNA binding domain of the gene 32 protein of the bacteriophage T4 contains a single "zinc-finger" sequence. The gene 32 protein is an extensively studied member of a class of proteins that bind relatively nonspecifically to single-stranded DNA. We have sequenced and characterized mutations in gene 32 whose defective proteins are activated by increasing the Zn(II) concentration in the growth medium. Our results identify a role for the gene 32 protein in activation of T4 late transcription. Several eukaryotic proteins with zinc fingers participate in activation of transcription, and the gene 32 protein of T4 should provide a simple, well-characterized system in which genetics can be utilized to study the role of a zinc finger in nucleic acid binding and gene expression.

MeSH Terms
DNA Mutational Analysis DNA Replication DNA, Single-Stranded/metabolism DNA-Binding Proteins/genetics,metabolism Gene Expression Regulation In Vitro Techniques Metalloproteins/genetics,metabolism Mutation T-Phages/physiology Transcription Factors/genetics,metabolism Transcription, Genetic Viral Proteins/genetics,metabolism Virus Replication Zinc/physiology
Chemicals
DNA, Single-Stranded DNA-Binding Proteins Metalloproteins Transcription Factors Viral Proteins Zinc
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Gauss P
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309.
Krassa K B
McPheeters D S
Nelson M A
Gold L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.23.8515
Published
1987-12-00
Pages
8515-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299575
Subset
IM
Grants
NIGMS NIH HHS · GM19963 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.23.8515 PMC Full Text View journal details
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