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PMID: 3119494 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A recombinant molecule from a disseminating strain of Neisseria gonorrhoeae that confers serum bactericidal resistance.

Infection and immunity ·Vol. 55 ·No. 12 ·1987-12-00 ·Pages 3017-22

McShan WM, Williams RP, Hull RA

Abstract

A cosmid gene library was prepared from Neisseria gonorrhoeae JC1, a serum-resistant clinical isolate from a patient with disseminated gonococcal infection. From this library a recombinant molecule, pWM3, was isolated which had the ability to transform F62, a serum-sensitive strain of N. gonorrhoeae, to serum resistance. This plasmid contained 2.2 kilobases of insert gonococcal DNA that coded for two peptides, one of 29 kilodaltons (kDa) and one of 17.5 kDa. Deletion of the region coding for the 29-kDa peptide resulted in the loss of the ability of the plasmid to transform F62 to serum resistance. N. gonorrhoeae F62 acquired the ability to bind blocking antibody when transformed with pWM3 or subclones that code for only the 29-kDa protein. Although similar in size, the cloned 29-kDa protein and protein III are antigenically distinct.

MeSH Terms
Antibodies, Bacterial/immunology Antigens, Bacterial/immunology Bacterial Proteins/immunology Blood Bactericidal Activity Cloning, Molecular Molecular Weight Neisseria gonorrhoeae/genetics,immunology Recombinant Proteins/immunology Transformation, Genetic
Chemicals
Antibodies, Bacterial Antigens, Bacterial Bacterial Proteins Recombinant Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
McShan W M
Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77030.
Williams R P
Hull R A
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.55.12.3017-3022.1987
Published
1987-12-00
Pages
3017-22
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260022
Subset
IM
Grants
NIAID NIH HHS · AI18462 · United States
NIAID NIH HHS · AI22851 · United States
External Links
PubMed source DOI: 10.1128/iai.55.12.3017-3022.1987 PMC Full Text View journal details
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