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PMID: 3119325 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Retroviral expression of the human IL-2 gene in a murine T cell line results in cell growth autonomy and tumorigenicity.

The EMBO journal ·Vol. 6 ·No. 9 ·1987-09-00 ·Pages 2705-9

Yamada G, Kitamura Y, Sonoda H, Harada H, Taki S, Mulligan RC, Osawa H, Diamantstein T, Yokoyama S, Taniguchi T

Abstract

In mature T lymphocytes (T cells) the regulated expression of the genes for interleukin-2 (IL-2) and its receptor (IL-2R) constitutes an essential part in controlling the cell growth. Evidence has been provided which suggests the involvement of an aberrant function of the IL-2 system in developing T cell neoplasms, particularly the adult T cell leukemia/lymphoma (ATL). As an approach to examine the extent of the IL-2 system contribution to T cell neoplasms, we created the experimental conditions wherein both IL-2 and IL-2R are expressed constitutively in a murine T cell line. We made use of a retroviral vector to infect an IL-2-dependent CTLL-2 line and lead to the expression of human IL-2. Here, we show that the virus-infected cells not only proliferate in vitro in the absence of exogenously supplied IL-2 under certain conditions, but also develop tumors (lymphomas) in nude and syngeneic mice.

MeSH Terms
Animals Cell Division Cell Line Cell Transformation, Neoplastic Genes Humans Interleukin-2/genetics Mice Moloney murine leukemia virus/genetics Plasmids Receptors, Immunologic/genetics Receptors, Interleukin-2 T-Lymphocytes/immunology Transfection
Chemicals
Interleukin-2 Receptors, Immunologic Receptors, Interleukin-2
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Yamada G
Institute for Molecular and Cellular Biology, Osaka University, Japan.
Kitamura Y
Sonoda H
Harada H
Taki S
Mulligan R C
Osawa H
Diamantstein T
Yokoyama S
Taniguchi T
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-09-00
Pages
2705-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553693
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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