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PMID: 3118144 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning of Bacillus subtilis genes involved in DNA metabolism.

Molecular & general genetics : MGG ·Vol. 209 ·No. 1 ·1987-08-00 ·Pages 8-14

Perego M, Ferrari E, Bassi MT, Galizzi A, Mazza P

Abstract

Different clones carrying a chromosomal DNA fragment able to transform Bacillus subtilis mutants dnaA13, dnaB19, dnaG5, recG40 and polA42 to a wild-type phenotype were isolated from a library constructed in plasmid pJH101. A lambda recombinant clone carrying a chromosomal fragment able to transform dnaC mutants was obtained from a lambda Charon 4A library. A restriction map of the cloned DNA fragments was constructed. The 11.3 kb cloned DNA fragment of plasmid pMP60-13 containing the wild-type sequence of dnaG5 was shown to transform a recF33 mutant as well.

MeSH Terms
Bacillus subtilis/genetics,metabolism Cloning, Molecular DNA, Bacterial/metabolism Genes Genes, Bacterial Genotype Mutation Plasmids
Chemicals
DNA, Bacterial
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Perego M
Dipartimento di Genetica e Microbiologia, A. Buzzati-Traverso, Università di Pavia, Italy.
Ferrari E
Bassi M T
Galizzi A
Mazza P
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00329829
Published
1987-08-00
Pages
8-14
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00329829
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