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PMID: 3117049 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Detection of 23-27 kDa GTP-binding proteins in platelets and other cells.

The Biochemical journal ·Vol. 245 ·No. 2 ·1987-07-15 ·Pages 617-20

Bhullar RP, Haslam RJ

Abstract

Membrane proteins from rabbit and human platelets were separated by SDS/polyacrylamide-gel electrophoresis and the resolved polypeptides blotted on nitrocellulose. A family of GTP-binding proteins, termed Gn proteins, was detected by incubation of these blots with [alpha-32P]GTP in the presence of Mg2+. A major Gn protein with a molecular mass of 27 kDa (Gn27) and lesser amounts of 23, 24 and 25 kDa Gn proteins were observed in platelet membranes; much smaller amounts were in the platelet soluble fraction. Binding of [alpha-32P]GTP by platelet Gn proteins was blocked by GDP, GTP or guanosine 5'-[gamma-thio]triphosphate, but not by GMP or adenosine 5'-[beta gamma-imido]triphosphate. Rabbit and human red-cell membranes contained only Gn27. When rat tissues were analysed for Gn proteins, the largest amounts were found in brain, which contained two membrane-bound forms (Gn27 and Gn26) and a soluble form (Gn26).

MeSH Terms
Animals Blood Platelets/metabolism Electrophoresis, Polyacrylamide Gel Erythrocyte Membrane/metabolism GTP-Binding Proteins/blood Guanosine Triphosphate/blood Humans Rabbits Rats Subcellular Fractions/metabolism Tissue Distribution
Chemicals
Guanosine Triphosphate GTP-Binding Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bhullar R P
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada.
Haslam R J
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2450617
Published
1987-07-15
Pages
617-20
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1148167
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2450617 PMC Full Text View journal details
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