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PMID: 3116043 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Plasminogen activator inhibitor is associated with the extracellular matrix of cultured bovine smooth muscle cells.

The Journal of clinical investigation ·Vol. 80 ·No. 4 ·1987-10-00 ·Pages 1082-9

Knudsen BS, Harpel PC, Nachman RL

Abstract

The extracellular matrix secreted by cultured bovine smooth muscle cells (BSMC) contains an endothelial type plasminogen activator (PA) inhibitor. When PA is incubated with the matrix, a high molecular weight complex containing a truncated PA inhibitor is released into the supernatant. The inhibitor also dissociates from the matrix by treatment with glycine, pH 2.7, in its intact, functionally active, 45-kD form, whereas treatment of the matrix with thrombin results in the release of a cleaved, inactive, 41 kD PA inhibitor. Bowes melanoma cells but not smooth muscle cells cultured on BSMC matrices decrease available matrix associated PA inhibitor. PA inhibitor incorporated into the extracellular matrix may serve an important role in the regulation of plasminogen activator mediated matrix degradation.

MeSH Terms
Animals Cattle Cells, Cultured Electrophoresis, Polyacrylamide Gel Extracellular Matrix/analysis Glycine/pharmacology Melanoma/metabolism Molecular Weight Muscle, Smooth/cytology Platelet Activating Factor/analysis Thrombin/metabolism Time Factors Tissue Plasminogen Activator/metabolism Urokinase-Type Plasminogen Activator/metabolism
Chemicals
Platelet Activating Factor Thrombin Tissue Plasminogen Activator Urokinase-Type Plasminogen Activator Glycine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Knudsen B S
Division of Hematology-Oncology, Cornell University Medical College, New York 10021.
Harpel P C
Nachman R L
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49 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI113164
Published
1987-10-00
Pages
1082-9
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC442350
Subset
IM
Grants
NHLBI NIH HHS · HL-18828 · United States
NHLBI NIH HHS · HL-30649 · United States
External Links
PubMed source DOI: 10.1172/JCI113164 PMC Full Text View journal details
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