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PMID: 3112522 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Genetic analysis of mannityl opine catabolism in octopine-type Agrobacterium tumefaciens strain 15955.

Molecular & general genetics : MGG ·Vol. 208 ·No. 1-2 ·1987-06-00 ·Pages 301-8

Dessaux Y, Tempé J, Farrand SK

Abstract

The genetic organization of functions responsible for mannityl opine catabolism of the Ti plasmid of Agrobacterium tumefaciens strain 1,5955 was investigated. A partial HindIII digest of pTi1,5955 was cloned into a broad host range cosmid and the clones obtained were tested for ability to confer mannityl opine degradation upon Agrobacterium. Inserts containing genes for catabolism of mannopinic acid, mannopine, agropine, and agropinic acid were obtained, spanning a segment of 43 kb on the Ti plasmid. Two clones conferring upon Agrobacterium the ability to catabolize the mannityl opines were mobilized to several Rhizobium sp., to Pseudomonas putida and P. fluorescens and to Escherichia coli. The catabolic functions were phenotypically expressed in all Rhizobium sp. tested, and in P. fluorescens, but not in P. putida or in E. coli.

MeSH Terms
Arginine/analogs & derivatives,biosynthesis Cloning, Molecular Cosmids Gene Expression Regulation Genes, Bacterial Lactones Mannitol/analogs & derivatives,metabolism Oxazines/metabolism Plasmids Rhizobium/genetics Species Specificity
Chemicals
Lactones Oxazines octopine Mannitol agropine agropinic acid mannopinic acid mannopine Arginine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dessaux Y
Tempé J
Farrand S K
References (18)
18 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330457
Published
1987-06-00
Pages
301-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NCI NIH HHS · R0I-CA19402 · United States
External Links
PubMed source DOI: 10.1007/BF00330457
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