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PMID: 311006 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Spontaneous and antibody-dependent cell-mediated cytotoxicity by human T cell subpopulations.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 10 ·1978-10-00 ·Pages 5137-41

Gupta S, Fernandes G, Nair M, Good RA

Abstract

Human peripheral blood non-T cells, T cells and their subpopulations (Tmu, Tgamma, Tphi, Tgamma-depleted cells, and Tmu-depleted cells) were assayed for their capacity to mediated spontaneous lymphocyte-mediated cytotoxicity (SLMC) or natural killer activity against K562 tumor cell line and antibody-dependent cellular cytotoxicity (ADCC) against chicken erythrocytes coated with antibody. Non-T cells, unseparated T cells, Tgamma cells, and Tmu-depleted (Tgamma-enriched) cells were found to have both SLMC (NK activity) and ADCC. Tmu, Tphi, and Tgamma-depleted cells had minimal or no SLMC and ADCC activity. This study demonstrates that SLMC and ADCC activity in T cells is mediated by Tgamma cell subpopulations. These two cytotoxic reactions were either mediated by two distinct subsets of Tgamma cells or by a single effector cell using two different mechanisms.

MeSH Terms
Antibody-Dependent Cell Cytotoxicity Cell Line Cytotoxicity, Immunologic Humans Leukemia, Myeloid/immunology T-Lymphocytes/immunology
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gupta S
Fernandes G
Nair M
Good R A
References (41)
41 references, click to expand
  1. Receptors for IgM are expressed on acute lymphoblastic leukemic cells having T-cell characteristics.
    Clin Immunol Immunopathol. 1977 May;7(3):405-9 PMID: 326452
  2. A functional comparison of human Fc-receptor-bearing lymphocytes active in natural cytotoxicity and antibody-dependent cellular cytotoxicity.
    J Immunol. 1977 Jun;118(6):2058-66 PMID: 325136
  3. Characterization of cytolytic effector cells in peripheral blood of healthy individuals and cancer patients. I. Surface markers and K cell activity after separation of B cells and lymphocytes and Fc-receptors by column fractionation.
    J Immunol. 1977 Jun;118(6):1919-24 PMID: 325129
  4. Loss of Fc receptors for IgG from human T lymphocytes exposed to IgG immune complexes.
    Nature. 1978 Apr 13;272(5654):618-20 PMID: 306068
  5. Low affinity E-rosette formation by the human K cell.
    J Immunol. 1978 Jan;120(1):90-5 PMID: 304874
  6. Characterization of the human natural killer (NK) cell in blood and lymphoid organs.
    Int J Cancer. 1978 Jan 15;21(1):42-50 PMID: 304845
  7. Subpopulations of human T lymphocytes. I. Studies in immunodeficient patients.
    Clin Exp Immunol. 1977 Nov;30(2):222-8 PMID: 304782
  8. Heterogeneity of locomotion in human T cell subsets.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2392-5 PMID: 307761
  9. Subpopulations of human T lymphocytes. III. Distribution and quantitation in peripheral blood, cord blood, tonsils, bone marrow, thymus, lymph nodes, and spleen.
    Cell Immunol. 1978 Mar 15;36(2):263-70 PMID: 305295
  10. Morphological and histochemical analyses of two human T-cell subpopulations bearing receptors for IgM or IgG.
    J Exp Med. 1978 May 1;147(5):1405-17 PMID: 306415
  11. Subpopulations of human T lymphocytes. IV. Quantitation and distribution in patients with mycosis fungoides and Sézary syndrome.
    Cell Immunol. 1978 Aug;39(1):18-26 PMID: 308846
  12. Functional analysis of two human T-cell subpopulations: help and suppression of B-cell responses by T cells bearing receptors for IgM or IgG.
    J Exp Med. 1977 Jul 1;146(1):184-200 PMID: 301544
  13. Natural cytotoxic reactivity of human lymphocytes against a myeloid cell line: characterization of effector cells.
    J Immunol. 1977 Jan;118(1):355-61 PMID: 299761
  14. Subpopulations of human T lymphocytes. II. Effect of thymopoietin, corticosteroids, and irradiation.
    Cell Immunol. 1977 Nov;34(1):10-8 PMID: 303151
  15. B cell antigens on effectors for natural cell-mediated cytotoxicity.
    Eur J Immunol. 1977 Oct;7(10):731-6 PMID: 304000
  16. Imbalances in T cell subpopulations associated with immunodeficiency and autoimmune syndromes.
    Eur J Immunol. 1977 Oct;7(10):696-700 PMID: 303998
  17. The use of Fab fragments of anti-human immunoglobulin as analytic tools for establishing the involvement of immunoglobulin in the spontaneous cytotoxicity to cultured tumor cells by lymphocytes from patients with bladder carcinoma and from healthy donors.
    J Immunol. 1977 Sep;119(3):1061-7 PMID: 302271
  18. Changes of Fcgamma receptor-related properties induced by interaction of human lymphocytes with insoluble immune complexes.
    J Immunol. 1977 Dec;119(6):1943-8 PMID: 562379
  19. Natural killing in immunodeficient patients.
    J Immunol. 1978 Mar;120(3):796-9 PMID: 632589
  20. Antibody-dependent cellular cytotoxicity in primary immunodeficiency diseases and with normal leukocyte subpopulations. Importance of the type of target.
    J Clin Invest. 1978 Jan;61(1):1-10 PMID: 618906
  21. Spontaneous human lymphocyte-mediated cytotoxicity against tumor target cells. II. Is the complement receptor necessarily present on the killer cells?
    Int J Cancer. 1977 Sep 15;20(3):353-8 PMID: 903188
  22. Spontaneous cell-mediated cytotoxicity by human peripheral blood lymphocytes in vitro.
    J Immunol. 1977 Oct;119(4):1401-5 PMID: 894045
  23. Loss of specific natural cell-mediated cytotoxicity with absorption of natural antibodies from serum.
    Int J Cancer. 1977 Jun 15;19(6):747-55 PMID: 873640
  24. Spontaneous human lymphocyte-mediated cytotoxicity againts tumour target cells. I. The effect of malignant disease.
    Int J Cancer. 1976 Nov 15;18(5):593-604 PMID: 825477
  25. Subpopulations of human T cells identified by receptors for immunoglobulins and mitogen responsiveness.
    J Immunol. 1976 Dec;117(6):2171-4 PMID: 792341
  26. Properties of the K562 cell line, derived from a patient with chronic myeloid leukemia.
    Int J Cancer. 1976 Oct 15;18(4):421-31 PMID: 789258
  27. The effector cells in human peripheral blood mediating mitogen-induced cellular cytotoxicity and antibody-dependent cellular cytotoxicity.
    J Immunol. 1976 Nov;117(5 Pt 1):1472-81 PMID: 1087314
  28. Immunocompetent cells in patients with malignant disease. I. The lack of naturally occurring killer cell activity in the unfractionated circulating lymphocytes from patients with chronic lymphocytic leukemia (CLL).
    Cancer. 1976 Dec;38(6):2274-7 PMID: 1000467
  29. Destruction of human lymphoid tissue-culture cell lines by human peripheral lymphocytes in 51Cr-release cellular cytotoxicity assays.
    J Natl Cancer Inst. 1974 Feb;52(2):345-52 PMID: 4131425
  30. Cytotoxic effects of lymphoid cells in vitro.
    Adv Immunol. 1969;11:117-93 PMID: 4392749
  31. Demonstration of cell-mediated immunity to human neoplasms of various histological types.
    Int J Cancer. 1971 Jan 15;7(1):1-16 PMID: 4322937
  32. Antibody-dependent cytotoxicity mediated by human Fc-receptor-bearing cells lacking markers for B- and T-lymphocytes.
    Int Arch Allergy Appl Immunol. 1974;47(1):139-54 PMID: 4603056
  33. Depression of cytotoxic T cell subpopulation in mice by hydrocortisone treatment.
    Clin Immunol Immunopathol. 1975 Jul;4(2):304-13 PMID: 237642
  34. Depressed lymphocyte-mediated killing of sensitized targets in cancer patients.
    Cancer Res. 1974 Oct;34(10):2694-8 PMID: 4412732
  35. Allogeneic cell-mediated testing for human tumor antigens.
    Natl Cancer Inst Monogr. 1972 Dec;35:251-7 PMID: 4659048
  36. Cytotoxic lymphocytes from normal donors. A functional marker of human non-T lymphocytes.
    Clin Exp Immunol. 1975 Aug;21(2):226-35 PMID: 810282
  37. Surface markers on human b and t lymphocytes. VI. Cytotoxicity against cell lines as a functional marker for lymphocyte subpopulations.
    Int J Cancer. 1975 Apr 15;15(4):596-605 PMID: 806545
  38. Antibody-dependent cell cytoxicity (ADCC). Characterization of 'killer' cells in human lymphoid organs.
    Scand J Immunol. 1976;5(3):233-42 PMID: 1084017
  39. The nature of the effector cell in antibody-dependent, cell-mediated cytolysis (ADCC): the cytotoxic activity of murine tumor cells and peritoneal macrophages.
    Clin Immunol Immunopathol. 1976 Mar;5(2):272-81 PMID: 1277588
  40. Temporary inhibition of antibody-dependent, cell-mediated cytotoxicity by pretreatment of human attacking cells with ammonium chloride.
    J Immunol. 1976 Apr;116(4):1170-2 PMID: 1254966
  41. Contactual lysis of antibody-coated chicken erythrocytes by purified lymphocytes.
    Cell Immunol. 1970 Sep;1(3):300-15 PMID: 5523583
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.10.5137
Published
1978-10-00
Pages
5137-41
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC336279
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.10.5137 PMC Full Text View journal details
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