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PMID: 3109388 Published · ppublish English Journal Article

Separation of multiple isomers of inositol phosphates formed in GH3 cells.

The Biochemical journal ·Vol. 242 ·No. 2 ·1987-03-01 ·Pages 361-6

Dean NM, Moyer JD

Abstract

A high-performance-liquid-chromatography (h.p.l.c.) separation was developed, which resolves isomers of inositol monophosphate (IP), inositol bisphosphate (IP2), and inositol trisphosphate (IP3) in a single run. In GH3 cells labelled with [3H]inositol, treated with Li+ and thyrotropin-releasing hormone (TRH), radiolabelled components identified as inositol 1-phosphate (I1P), inositol 2-phosphate (I2P), inositol 4-phosphate (I4P), inositol 1,4-bisphosphate [I(1,4)P2], inositol 1,3,4-trisphosphate [I(1,3,4)P3] and inositol 1,4,5-trisphosphate [I(1,4,5)P3] are present, as are multiple unidentified IP2 peaks. After TRH stimulation, both I1P and I4P increase, the increase in I4P preceding that of I1P; I(1,4)P2 and an unknown IP2 increase; and both I(1,3,4)P3 and I(1,4,5)P3 increase, the increase in I(1,4,5)P3 being rapid and transient, whereas the increase in I(1,3,4)P3 is slower and more sustained. The most rapidly appearing inositol phosphates produced after TRH stimulation are I(1,4)P2 and I(1,4,5)P3.

MeSH Terms
Cell Line Chromatography, High Pressure Liquid Inositol Phosphates/isolation & purification,metabolism Isomerism Kinetics Sugar Phosphates/isolation & purification Thyrotropin-Releasing Hormone/pharmacology
Chemicals
Inositol Phosphates Sugar Phosphates Thyrotropin-Releasing Hormone
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dean N M
Moyer J D
References (28)
28 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2420361
Published
1987-03-01
Pages
361-6
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1147713
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2420361 PMC Full Text View journal details
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