Home LiteratureArticle Details
PMID: 3106215 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Probing the surface of Neisseria gonorrhoeae: simultaneous localization of protein I and H.8 antigens.

Infection and immunity ·Vol. 55 ·No. 5 ·1987-05-00 ·Pages 1190-7

Robinson EN, McGee ZA, Buchanan TM, Blake MS, Hitchcock PJ

Abstract

Gonococcal outer membrane protein I and the neisserial antigen H.8 are being investigated for inclusion in a gonococcal vaccine. To determine the distribution of immunoaccessible protein I and H.8 molecules on the surface of viable gonococci and to approximate the accessibility of these antigens to vaccine-elicited antibodies, immunologic probes composed of protein I- and H.8-specific antibodies linked to gold spheres were developed. When whole gonococci were exposed to the protein I and H.8 immunologic probes and examined by transmission electron microscopy, gold spheres clearly marked the surface of some of the gonococci, but not the surface of other gonococci from the same culture. The immunologic accessibility of gonococcal protein I or neisserial H.8 varied among gonococci. This diversity may affect the efficacy of a vaccine composed of these surface antigens.

MeSH Terms
Antibodies, Monoclonal Antigens, Bacterial/isolation & purification Antigens, Surface/isolation & purification Bacterial Outer Membrane Proteins/isolation & purification Bacterial Vaccines/isolation & purification Gold Gonorrhea/prevention & control Humans Neisseria gonorrhoeae/immunology Staphylococcal Protein A
Chemicals
Antibodies, Monoclonal Antigens, Bacterial Antigens, Surface Bacterial Outer Membrane Proteins Bacterial Vaccines Staphylococcal Protein A Gold
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Robinson E N
McGee Z A
Buchanan T M
Blake M S
Hitchcock P J
References (35)
35 references, click to expand
  1. Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3116-20 PMID: 91164
  2. Antigen-specific serotyping of Neisseria gonorrhoeae: characterization based upon principal outer membrane protein.
    Infect Immun. 1981 Jun;32(3):985-94 PMID: 6166568
  3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  4. Surface-exposed protein antigens of the gonococcal outer membrane.
    Infect Immun. 1981 Dec;34(3):804-16 PMID: 6800957
  5. Bacteriophage-resistant mutants of Salmonella typhimurium deficient in two major outer membrane proteins.
    J Bacteriol. 1976 Aug;127(2):941-55 PMID: 783123
  6. Properties of the major outer membrane protein from Neisseria gonorrhoeae incorporated into model lipid membranes.
    Proc Natl Acad Sci U S A. 1983 Jun;80(12):3831-5 PMID: 6407021
  7. NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION.
    J Bacteriol. 1963 Jun;85:1274-9 PMID: 14047217
  8. Nonpilar surface appendages of colony type 1 and colony type 4 gonococci.
    Infect Immun. 1976 Jul;14(1):266-70 PMID: 820643
  9. Monoclonal antibody that recognizes an outer membrane antigen common to the pathogenic Neisseria species but not to most nonpathogenic Neisseria species.
    Infect Immun. 1984 Mar;43(3):994-9 PMID: 6421742
  10. Antigenicity of Neisseria gonorrhoeae outer membrane protein(s) III detected by immunoprecipitation and Western blot transfer with a monoclonal antibody.
    Infect Immun. 1982 Nov;38(2):668-72 PMID: 6183218
  11. Effects of proteolytic enzymes on the outer membrane proteins of Neisseria gonorrhoeae.
    Infect Immun. 1981 Jul;33(1):212-22 PMID: 6790441
  12. Ultrastructural localization of specific gonococcal macromolecules with antibody-gold sphere immunological probes.
    Infect Immun. 1984 Nov;46(2):361-6 PMID: 6150005
  13. Surface peptide mapping of protein I and protein III of four strains of Neisseria gonorrhoeae.
    Infect Immun. 1982 Aug;37(2):632-41 PMID: 6811436
  14. A pilus peptide vaccine for the prevention of gonorrhea.
    Prog Allergy. 1983;33:314-31 PMID: 6187017
  15. Purification and partial characterization of the major outer membrane protein of Neisseria gonorrhoeae.
    Infect Immun. 1982 Apr;36(1):277-83 PMID: 6804388
  16. Variable major proteins of Borrellia hermsii.
    J Exp Med. 1982 Nov 1;156(5):1312-24 PMID: 7130901
  17. Neisseria gonorrhoeae. II. Colonial variation and pathogenicity during 35 months in vitro.
    J Bacteriol. 1968 Sep;96(3):596-605 PMID: 4979098
  18. O-antigenic chains of lipopolysaccharide prevent binding of antibody molecules to an outer membrane pore protein in Enterobacteriaceae.
    Microb Pathog. 1986 Feb;1(1):43-9 PMID: 2469929
  19. Studies of Porins: Spontaneously Transferred from Whole Cells and Reconstituted from Purified Proteins of Neisseria gonorrhoeae and Neisseria meningitidis.
    Biophys J. 1984 Jan;45(1):104-7 PMID: 19431528
  20. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  21. A new method of preparing gold probes for multiple-labeling cytochemistry.
    Eur J Cell Biol. 1985 Jul;38(1):87-93 PMID: 4029177
  22. Serology of Neisseria gonorrhoeae: W-antigen serogrouping by coagglutination and protein I serotyping by enzyme-linked immunosorbent assay both detect protein I antigens.
    Infect Immun. 1982 Jan;35(1):229-39 PMID: 6172380
  23. Studies on gonococcus infection. XII. Colony color and opacity varienats of gonococci.
    Infect Immun. 1978 Jan;19(1):320-31 PMID: 415006
  24. Confirmation of association of protein I serotype of Neisseria gonorrhoeae with ability to cause disseminated infection.
    Infect Immun. 1983 May;40(2):816-9 PMID: 6404835
  25. Serological classification of Neisseria gonorrhoeae with monoclonal antibodies.
    Infect Immun. 1982 Jun;36(3):1042-53 PMID: 6807844
  26. Gonococcal membrane proteins: speculation on their role in pathogenesis.
    Prog Allergy. 1983;33:298-313 PMID: 6828470
  27. Analyses of gonococcal H8 antigen. Surface location, inter- and intrastrain electrophoretic heterogeneity, and unusual two-dimensional electrophoretic characteristics.
    J Exp Med. 1985 Dec 1;162(6):2017-34 PMID: 2415662
  28. Monoclonal antibody activity against native and denatured forms of gonococcal outer membrane proteins as detected within ultrathin, longitudinal slices of polyacrylamide gels.
    J Immunol Methods. 1984 Dec 31;75(2):265-74 PMID: 6084031
  29. Diffusion of aminoglycoside antibiotics across the outer membrane of Escherichia coli.
    Antimicrob Agents Chemother. 1982 Oct;22(4):554-9 PMID: 6758685
  30. Analyses of gonococcal lipopolysaccharide in whole-cell lysates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis: stable association of lipopolysaccharide with the major outer membrane protein (protein I) of Neisseria gonorrhoeae.
    Infect Immun. 1984 Oct;46(1):202-12 PMID: 6207109
  31. Neisserial antigen H.8 is immunogenic in patients with disseminated gonococcal and meningococcal infections.
    J Infect Dis. 1985 Apr;151(4):650-7 PMID: 3919117
  32. Proteins IA and IB exhibit different surface exposures and orientations in the outer membranes of Neisseria gonorrhoeae.
    Infect Immun. 1984 Jun;44(3):565-8 PMID: 6427111
  33. The surface properties of Neisseria gonorrhoeae: topographical distribution of the outer membrane protein antigens.
    J Gen Microbiol. 1978 Oct;108(2):213-9 PMID: 102733
  34. Mutants of Escherichia coli K12 lacking all 'major' proteins of the outer cell envelope membrane.
    FEBS Lett. 1975 Jul 15;55(1):161-4 PMID: 806476
  35. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels.
    J Bacteriol. 1983 Apr;154(1):269-77 PMID: 6187729
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.55.5.1190-1197.1987
Published
1987-05-00
Pages
1190-7
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260489
Subset
IM
Grants
NIAID NIH HHS · AI-20265 · United States
External Links
PubMed source DOI: 10.1128/iai.55.5.1190-1197.1987 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2