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PMID: 3104404 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Gamma-interferon inhibits collagen synthesis in vivo in the mouse.

The Journal of clinical investigation ·Vol. 79 ·No. 4 ·1987-04-00 ·Pages 1254-8

Granstein RD, Murphy GF, Margolis RJ, Byrne MH, Amento EP

Abstract

Subcutaneous implantation of osmotic pumps into CAF1 mice resulted in the formation of thick fibrous capsules around the pumps. When pumps were loaded with recombinant murine gamma-interferon (rMuIFN-gamma) to deliver 2 X 10(3) U/h for 14 d, there was a marked decrease in thickness and collagen content of the capsules from rMuIFN-gamma-treated animals compared with capsules from animals receiving diluent alone. The collagen content of the capsules was estimated by hydroxyproline analysis of the tissue and by quantitative electron microscopy of collagen bundles. Heat-inactivated rMuIFN-gamma failed to reduce the fibrotic response in this assay. These results provide compelling evidence that gamma-interferon can down-regulate collagen synthesis in vivo and suggest the possibility that this lymphokine may be useful in the treatment of disease states characterized by excessive fibrosis.

MeSH Terms
Animals Collagen/biosynthesis Female Hydroxyproline/analysis Infusion Pumps Interferon-gamma/administration & dosage,pharmacology Mice Microscopy, Electron
Chemicals
Interferon-gamma Collagen Hydroxyproline
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Granstein R D
Murphy G F
Margolis R J
Byrne M H
Amento E P
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36 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI112945
Published
1987-04-00
Pages
1254-8
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC424325
Subset
IM
Grants
NIADDK NIH HHS · AM-01425-02 · United States
NIADDK NIH HHS · AM-03564 · United States
External Links
PubMed source DOI: 10.1172/JCI112945 PMC Full Text View journal details
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