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PMID: 3099773 Published · ppublish English Journal Article

Ionophore A23187 induces a refractory state in thrombin-activated release of inositol phosphates.

The Biochemical journal ·Vol. 238 ·No. 3 ·1986-09-15 ·Pages 709-14

Moscat G, Moreno F, Iglesias S, Garcia-Barreno P, Municio AM

Abstract

The phosphatidylinositol cycle has been proposed to be involved in the regulation of platelet functionality through the control of cytoplasmic Ca2+ levels. However, the requirements of phospholipase C for Ca2+ has not yet been elucidated in intact platelets. The primary purpose of the present study was to investigate the Ca2+ requirements of this enzyme in platelets from miniature swine by taking advantage of the permeabilizing properties of the ionophore A23187. Our results strongly suggest that the treatment of platelets with A23187 induces a refractory state in thrombin-stimulated release of inositol phosphates while 5-hydroxytryptamine (serotonin)-secretory capacity in response to thrombin remained constant. This refractory state seems to be dependent on some cytochalasin-inhibitable cytoskeletal phenomena.

MeSH Terms
Animals Blood Platelets/drug effects,metabolism Calcimycin/pharmacology Calcium/pharmacology Cytochalasin B/pharmacology Inositol Phosphates/blood Serotonin/blood Sugar Phosphates/blood Swine Thrombin/pharmacology Type C Phospholipases/blood
Chemicals
Inositol Phosphates Sugar Phosphates Serotonin Calcimycin Cytochalasin B Type C Phospholipases Thrombin Calcium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Moscat G
Moreno F
Iglesias S
Garcia-Barreno P
Municio A M
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34 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2380709
Published
1986-09-15
Pages
709-14
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1147195
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2380709 PMC Full Text View journal details
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