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PMID: 309941 Published · ppublish English Journal Article

Inward calcium current in twitch muscle fibres of the frog.

The Journal of physiology ·Vol. 283 ·1978-10-00 ·Pages 197-209

Sanchez JA, Stefani E

Abstract

1. Voltage clamp experiments using the three micro-electrode voltage clamp technique were performed on sartorius muscles of the frog. 2. By blocking potassium currents with tetraethylammonium and replacing chloride ions with sulphate a slow inward current was detected. 3. The slow inward current is mainly carried by calcium, since it is abolished by cobalt and D-600, it depends on external calcium, and is not affected by removing external sodium or by tetrodotoxin (TTX). 4. The slow inward current has a mean threshold of -40 mV, reaches a mean maximum value at ca. 0 mV of 81 microamperemetercm-2 and has a mean reversal potential of +38 mV. 5. The calcium current is inactivated by the application of 2 sec conditioning prepulses according to a sigmoid curve with V(h) = -42 mV and k = 6.2 mV. 6. The slow time course of this calcium current makes it rather unlikely that it participates in contraction during a twitch, but it might be activated during long depolarizations as potassium contractures.

MeSH Terms
Action Potentials Animals Anura Calcium/physiology Cobalt/pharmacology Electric Conductivity Gallopamil/pharmacology In Vitro Techniques Membrane Potentials/drug effects Muscles/physiology Rana pipiens Sodium/pharmacology Tetraethylammonium Compounds/pharmacology Tetrodotoxin/pharmacology
Chemicals
Tetraethylammonium Compounds Gallopamil Cobalt Tetrodotoxin Sodium Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sanchez J A
Stefani E
References (36)
36 references, click to expand
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Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.1978.sp012496
Published
1978-10-00
Pages
197-209
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1282773
Subset
IM
External Links
PubMed source DOI: 10.1113/jphysiol.1978.sp012496 PMC Full Text View journal details
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