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PMID: 3098687 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of protein I from serum-sensitive and serum-resistant transformants of Neisseria gonorrhoeae.

Infection and immunity ·Vol. 55 ·No. 1 ·1987-01-00 ·Pages 273-6

Judd RC, Tam M, Joiner K

Abstract

The protein IAs of serum-sensitive (FA635) and serum-resistant (FA638) transformants of Neisseria gonorrhoeae, which have identical pedigrees, have been shown to be different by the use of a monoclonal antibody and were also shown to be different by proteinase K cleavage and primary structural and surface peptide mapping. The difference in structure is within the surface-exposed region of the molecule. The only other difference observed between the two strains was a very slight difference in lipooligosaccharide silver staining in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These data suggest that protein I alone or in combination with lipooligosaccharide may significantly contribute to serum resistance.

MeSH Terms
Antigens, Bacterial/analysis Bacterial Proteins/immunology Lipopolysaccharides/immunology Molecular Weight Neisseria gonorrhoeae/immunology Peptide Fragments/analysis
Chemicals
Antigens, Bacterial Bacterial Proteins Lipopolysaccharides Peptide Fragments lipid-linked oligosaccharides
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Judd R C
Tam M
Joiner K
References (19)
19 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.55.1.273-276.1987
Published
1987-01-00
Pages
273-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260316
Subset
IM
Grants
NIAID NIH HHS · 3RO1-AI21236-02S1 · United States
NIAID NIH HHS · 5RO1-AI21236-02 · United States
External Links
PubMed source DOI: 10.1128/iai.55.1.273-276.1987 PMC Full Text View journal details
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