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PMID: 3096727 Published · ppublish English Journal Article

Modulation of adherence of coagulase-negative staphylococci to Teflon catheters in vitro.

European journal of clinical microbiology ·Vol. 5 ·No. 5 ·1986-10-00 ·Pages 518-22

Pascual A, Fleer A, Westerdaal NA, Verhoef J

Abstract

The mechanism of adherence of Staphylococcus epidermidis to commercially available catheters was studied in vitro in a quantitative assay employing 3H-labelled bacteria. It was found that adherence to Teflon catheters was significantly related to the degree of hydrophobicity of the strains. When hydrophobic groups were removed from Staphylococcus epidermidis by pepsin treatment, adhesion was almost completely abolished. Preincubation of catheters in human serum also caused a 80-90% reduction of adherence. Preincubation of Staphylococcus epidermidis in serum similarly decreased adhesion. This effect of serum was mainly due to albumin, while IgG and fibronectin were less effective. Culture of Staphylococcus epidermidis in subinhibitory concentrations (0.5 MIC) of cephalothin, clindamycin and vancomycin resulted in a 30-80% reduction in adhesion.

MeSH Terms
Anti-Bacterial Agents/pharmacology Bacterial Adhesion Blood Proteins/pharmacology Catheters, Indwelling Coagulase Fibronectins/pharmacology Humans Immunoglobulin G/pharmacology Infant, Newborn Kinetics Pepsin A/pharmacology Polytetrafluoroethylene/metabolism Staphylococcal Infections/prevention & control Staphylococcus epidermidis/metabolism
Chemicals
Anti-Bacterial Agents Blood Proteins Coagulase Fibronectins Immunoglobulin G Polytetrafluoroethylene Pepsin A
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pascual A
Fleer A
Westerdaal N A
Verhoef J
References (19)
19 references, click to expand
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Article Info
Journal
European journal of clinical microbiology
Abbr.
Eur J Clin Microbiol
ISSN
0722-2211
DOI
10.1007/BF02017694
Published
1986-10-00
Pages
518-22
Language
English
Region
Germany
NLM ID
8219582
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02017694
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