Home LiteratureArticle Details
PMID: 3095335 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Talin at myotendinous junctions.

The Journal of cell biology ·Vol. 103 ·No. 4 ·1986-10-00 ·Pages 1465-72

Tidball JG, O'Halloran T, Burridge K

Abstract

Junctions formed by skeletal muscles where they adhere to tendons, called myotendinous junctions, are sites of tight adhesion and where forces generated by the cell are placed on the substratum. In this regard, myotendinous junctions and focal contacts of fibroblasts in vitro are analogues. Talin is a protein located at focal contacts that may be involved in force transmission from actin filaments to the plasma membrane. This study investigates whether talin is also found at myotendinous junctions. Protein separations on SDS polyacrylamide gels and immunolabeling procedures show that talin is present in skeletal muscle. Immunofluorescence microscopy using anti-talin indicates that talin is found concentrated at myotendinous junctions and in lesser amounts in periodic bands over nonjunctional regions. Electron microscopic immunolabeling shows talin is a component of the digitlike processes of muscle cells that extend into tendons at myotendinous junctions. These findings indicate that there may be similarities in the molecular composition of focal contacts and myotendinous junctions in addition to functional analogies.

MeSH Terms
Animals Chickens Cytoskeletal Proteins/analysis,physiology Fluorescent Antibody Technique Intercellular Junctions/analysis,ultrastructure Muscles/ultrastructure Stress, Mechanical Talin Tendons/ultrastructure
Chemicals
Cytoskeletal Proteins Talin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tidball J G
O'Halloran T
Burridge K
References (52)
52 references, click to expand
  1. Microfilament bundles and cell shape are related to adhesiveness to substratum and are dissociable from growth control in cultured fibroblasts.
    Cell. 1977 Mar;10(3):375-80 PMID: 557370
  2. Electron microscopic study of alpha-actinin.
    J Mol Biol. 1975 Feb 25;92(2):357-9 PMID: 1142428
  3. Vinculin, an intracellular protein localized at specialized sites where microfilament bundles terminate at cell membranes.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):4127-31 PMID: 6776523
  4. Distribution of the cell substratum attachment (CSAT) antigen on myogenic and fibroblastic cells in culture.
    J Cell Biol. 1985 May;100(5):1528-39 PMID: 3921554
  5. Interaction of cytoskeletal proteins on the human erythrocyte membrane.
    Cell. 1981 Apr;24(1):24-32 PMID: 6453651
  6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  7. Identification and organization of the components in the isolated microvillus cytoskeleton.
    J Cell Biol. 1979 Dec;83(3):667-73 PMID: 574874
  8. Properties of smooth muscle vinculin.
    J Biol Chem. 1984 Mar 25;259(6):3916-24 PMID: 6323447
  9. The molecular basis for membrane - cytoskeleton association in human erythrocytes.
    J Cell Biochem. 1982;18(1):49-65 PMID: 6461664
  10. Purification of a 190 kDa protein from smooth muscle: relationship to talin.
    Biochim Biophys Acta. 1986 Feb 14;869(3):337-49 PMID: 2418880
  11. Deficient red-cell spectrin in severe, recessively inherited spherocytosis.
    N Engl J Med. 1982 May 13;306(19):1155-61 PMID: 7070419
  12. Myotendinous junctions of tonic muscle cells: structure and loading.
    Cell Tissue Res. 1986;245(2):315-22 PMID: 3488810
  13. Immunocytochemical localization of 140 kD cell adhesion molecules in cultured chicken fibroblasts, and in chicken smooth muscle and intestinal epithelial tissues.
    J Histochem Cytochem. 1985 Jun;33(6):576-86 PMID: 3889142
  14. A vinculin-containing cortical lattice in skeletal muscle: transverse lattice elements ("costameres") mark sites of attachment between myofibrils and sarcolemma.
    Proc Natl Acad Sci U S A. 1983 Feb;80(4):1008-12 PMID: 6405378
  15. A 130K protein from chicken gizzard: its localization at the termini of microfilament bundles in cultured chicken cells.
    Cell. 1979 Sep;18(1):193-205 PMID: 574428
  16. Organization and function of stress fibers in cells in vitro and in situ. A review.
    Cell Muscle Motil. 1984;5:83-137 PMID: 6367964
  17. Brush border cytoskeleton and integration of cellular functions.
    J Cell Biol. 1984 Jul;99(1 Pt 2):104s-112s PMID: 6378918
  18. The behaviour of fibroblasts migrating from chick heart explants: changes in adhesion, locomotion and growth, and in the distribution of actomyosin and fibronectin.
    J Cell Sci. 1979 Oct;39:149-65 PMID: 575139
  19. Marked reduction of spectrinin hereditary spherocytosis in the common house mouse.
    Blood. 1978 Jun;51(6):1149-55 PMID: 647119
  20. Talin is a post-synaptic component of the rat neuromuscular junction.
    Exp Cell Res. 1986 Mar;163(1):143-50 PMID: 3080319
  21. Are stress fibres contractile?
    Nature. 1981 Dec 24;294(5843):691-2 PMID: 7198718
  22. Interaction of plasma membrane fibronectin receptor with talin--a transmembrane linkage.
    Nature. 1986 Apr 10-16;320(6062):531-3 PMID: 2938015
  23. Widespread occurrence of avian spectrin in nonerythroid cells.
    Cell. 1982 Jul;29(3):821-33 PMID: 6758951
  24. Spectrin-actin membrane skeleton of normal and abnormal red blood cells.
    Semin Hematol. 1979 Jan;16(1):21-51 PMID: 370983
  25. Interaction of alpha-actinin and vinculin with actin: opposite effects on filament network formation.
    Proc Natl Acad Sci U S A. 1981 May;78(5):3005-9 PMID: 6789327
  26. The geometry of actin filament-membrane associations can modify adhesive strength of the myotendinous junction.
    Cell Motil. 1983;3(5-6):439-47 PMID: 6607113
  27. Immunoprecipitation of nonerythrocyte spectrin within live cells following microinjection of specific antibodies: relation to cytoskeletal structures.
    J Cell Biol. 1984 Apr;98(4):1363-77 PMID: 6371021
  28. Actin-membrane interaction in fibroblasts: what proteins are involved in this association?
    J Cell Biol. 1984 Jul;99(1 Pt 2):95s-103s PMID: 6430913
  29. Alpha-actinin: immunofluorescent localization of a muscle structural protein in nonmuscle cells.
    Cell. 1975 Nov;6(3):289-98 PMID: 802682
  30. A rapid purification of alpha-actinin, filamin, and a 130,000-dalton protein from smooth muscle.
    J Biol Chem. 1980 Feb 10;255(3):1194-9 PMID: 7356657
  31. Structural connections of the muscle-tendon junction.
    Cell Motil. 1983;3(5-6):431-8 PMID: 6686490
  32. Preparation of iodine-131 labelled human growth hormone of high specific activity.
    Nature. 1962 May 5;194:495-6 PMID: 14450081
  33. The spectrin membrane skeleton of normal and abnormal human erythrocytes: a review.
    Am J Physiol. 1983 Mar;244(3):C121-41 PMID: 6338732
  34. Molecular heterogeneity of adherens junctions.
    J Cell Biol. 1985 Oct;101(4):1523-31 PMID: 3930512
  35. Interactions of normal and neoplastic fibroblasts with the substratum.
    Ciba Found Symp. 1973;14:311-31 PMID: 4360365
  36. Microfilament organization in the cytoskeleton of the intestinal brush border.
    Cell Muscle Motil. 1983;4:239-68 PMID: 6367959
  37. Non-muscle alpha actinins are calcium-sensitive actin-binding proteins.
    Nature. 1981 Dec 10;294(5841):565-7 PMID: 7312045
  38. Talin: a cytoskeletal component concentrated in adhesion plaques and other sites of actin-membrane interaction.
    Cell Motil. 1983;3(5-6):405-17 PMID: 6319001
  39. Silicone rubber substrata: a new wrinkle in the study of cell locomotion.
    Science. 1980 Apr 11;208(4440):177-9 PMID: 6987736
  40. Development of cell surface linkage complexes in cultured fibroblasts.
    J Cell Biol. 1985 Apr;100(4):1103-14 PMID: 3884631
  41. Vinculin in subsarcolemmal densities in chicken skeletal muscle: localization and relationship to intracellular and extracellular structures.
    J Cell Biol. 1985 Jul;101(1):240-56 PMID: 3924918
  42. Relation between cell activity and the distribution of cytoplasmic actin and myosin.
    J Cell Biol. 1981 Jul;90(1):84-91 PMID: 7019223
  43. Effects of abnormal cytoskeletal structure on erythrocyte membrane mechanical properties.
    Cell Motil. 1983;3(5-6):609-22 PMID: 6686495
  44. Structure and function of the murine muscle-tendon junction.
    Anat Rec. 1981 Oct;201(2):293-302 PMID: 7198392
  45. The cell substrate attachment (CSAT) antigen has properties of a receptor for laminin and fibronectin.
    J Cell Biol. 1985 Dec;101(6):2134-44 PMID: 2933413
  46. Cytoskeletal components of the vertebrate neuromuscular junction: vinculin, alpha-actinin, and filamin.
    J Cell Biol. 1983 Jul;97(1):217-23 PMID: 6408100
  47. Selective enzyme purification by affinity chromatography.
    Proc Natl Acad Sci U S A. 1968 Oct;61(2):636-43 PMID: 4971842
  48. An interaction between vinculin and talin.
    Nature. 1984 Apr 19-25;308(5961):744-6 PMID: 6425696
  49. A new protein of adhesion plaques and ruffling membranes.
    J Cell Biol. 1983 Aug;97(2):359-67 PMID: 6684120
  50. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.
    Anal Biochem. 1981 Apr;112(2):195-203 PMID: 6266278
  51. Myotendinous junction: morphological changes and mechanical failure associated with muscle cell atrophy.
    Exp Mol Pathol. 1984 Feb;40(1):1-12 PMID: 6607176
  52. Heterogeneity of spectrin distribution among avian muscle fiber types.
    Muscle Nerve. 1984 Jun;7(5):408-14 PMID: 6377061
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.103.4.1465
Published
1986-10-00
Pages
1465-72
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114324
Subset
IM
Grants
NIGMS NIH HHS · GM-29860 · United States
External Links
PubMed source DOI: 10.1083/jcb.103.4.1465 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2