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PMID: 3085098 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transformation of the cyanobacterium Anacystis nidulans 6301 with the Escherichia coli plasmid pBR322.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 8 ·1986-04-00 ·Pages 2546-50

Daniell H, Sarojini G, McFadden BA

Abstract

Anacystis nidulans 6301 has been transformed in the light to ampicillin resistance with the plasmid pBR322. Permeaplasts prepared by 2-hr treatment of cells with lysozyme and EDTA are transformed with a 50-fold higher efficiency than that observed for cells. beta-Lactamase is present in A. nidulans transformed either with pBR322 or the plasmid pCH1 as evidenced by hydrolysis of the beta-lactam ring of Nitrocefin in extracts of transformants. beta-Lactamase also can be immunoprecipitated from extracts of [35S]methionine-labeled pBR322 transformants and coprecipitates with ribulose-bisphosphate carboxylase. Expression of the carboxylase is apparently amplified in pBR322 transformants as is that for several soluble proteins in pCH1 transformants. Chromosomal DNA per cell is increased about 6-fold after transformation of A. nidulans 6301 with either pBR322 or pCH1. A 4.3-kilobase-pair plasmid can be isolated from pBR322 transformants in addition to the endogenous plasmids pUH24 and pUH25.

MeSH Terms

Cell Membrane Permeability Cyanobacteria/genetics DNA, Bacterial/genetics Escherichia coli/genetics Genetic Vectors Plasmids Transformation, Genetic beta-Lactamases/genetics

Chemicals

DNA, Bacterial beta-Lactamases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Daniell H

Sarojini G

McFadden B A

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25 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.83.8.2546

Published

1986-04-00

Pages

2546-50

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC323335

Subset

Grants

NIGMS NIH HHS · GM19972 · United States

External Links

PubMed source DOI: 10.1073/pnas.83.8.2546 PMC Full Text View journal details

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Transformation of the cyanobacterium Anacystis nidulans 6301 with the Escherichia coli plasmid pBR322.

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