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PMID: 30761122 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

LAYN Is a Prognostic Biomarker and Correlated With Immune Infiltrates in Gastric and Colon Cancers.

Frontiers in immunology ·Vol. 10 ·2019-00-00 ·Pages 6

Pan JH, Zhou H, Cooper L, Huang JL, Zhu SB, Zhao XX, Ding H, Pan YL, Rong L

Abstract

Background: Layilin (LAYN) is a critical gene that regulates T cell function. However, the correlations of LAYN to prognosis and tumor-infiltrating lymphocytes in different cancers remain unclear. Methods: LAYN expression was analyzed via the Oncomine database and Tumor Immune Estimation Resource (TIMER) site. We evaluated the influence of LAYN on clinical prognosis using Kaplan-Meier plotter, the PrognoScan database and Gene Expression Profiling Interactive Analysis (GEPIA). The correlations between LAYN and cancer immune infiltrates was investigated via TIMER. In addition, correlations between LAYN expression and gene marker sets of immune infiltrates were analyzed by TIMER and GEPIA. Results: A cohort (GSE17536) of colorectal cancer patients showed that high LAYN expression was associated with poorer overall survival (OS), disease-specific survival (DSS), and disease-free survival (DFS). In addition, high LAYN expression was significantly correlated with poor OS and progression-free survival (PFS) in gastric cancers (OS HR = 1.97, P = 3.6e-10; PFS HR = 2.12, P = 2.3e-10). Moreover, LAYN significantly impacts the prognosis of diverse cancers via The Cancer Genome Atlas (TCGA). Specifically, high LAYN expression was correlated with worse OS and PFS in stage 2 to 4 but not stage 1 and stage N0 gastric cancer patients (P = 0.28, 0.34; P = 0.073, 0.092). LAYN expression was positively correlated with infiltrating levels of CD4+ T and CD8+ T cells, macrophages, neutrophils, and dendritic cells (DCs) in colon adenocarcinoma (COAD) and stomach adenocarcinoma (STAD). LAYN expression showed strong correlations with diverse immune marker sets in COAD and STAD. Conclusions: These findings suggest that LAYN is correlated with prognosis and immune infiltrating levels of, including those of CD8+ T cells, CD4+ T cells, macrophages, neutrophils, and DCs in multiple cancers, especially in colon and gastric cancer patients. In addition, LAYN expression potentially contributes to regulation of tumor-associated macrophages (TAMs), DCs, T cell exhaustion and Tregs in colon and gastric cancer. These findings suggest that LAYN can be used as a prognostic biomarker for determining prognosis and immune infiltration in gastric and colon cancers.

Keywords

LAYN colon cancer gastric cancer lymphocytes prognosis tumor-infiltrating

MeSH Terms

Biomarkers, Tumor Colonic Neoplasms/etiology,mortality,pathology Databases, Factual Dendritic Cells/immunology,metabolism,pathology Female Gene Expression Profiling Humans Kaplan-Meier Estimate Lectins, C-Type/genetics,metabolism Lymphocyte Subsets/immunology,metabolism Lymphocytes, Tumor-Infiltrating/immunology,metabolism,pathology Macrophages/immunology,metabolism,pathology Male Neoplasm Metastasis Neoplasm Staging Prognosis Survival Analysis Tumor Microenvironment/immunology

Chemicals

Biomarkers, Tumor LAYN protein, human Lectins, C-Type

Authors & Affiliations

9 authors, click to expand affiliations / ORCID

Pan Jing-Hua

Department of General Surgery, The First Affiliated Hospital of Jinan University, Guangzhou, China. | Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, Chicago, IL, United States.

Zhou Hong

Department of Gynecology, The First Affiliated Hospital of Jinan University, Guangzhou, China.

Cooper Laura

Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, Chicago, IL, United States.

Huang Jin-Lian