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PMID: 3071737 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Killing of Escherichia coli cells modulated by components of the stability system ParD of plasmid R1.

Molecular & general genetics : MGG ·Vol. 215 ·No. 1 ·1988-12-00 ·Pages 146-51

Bravo A, Ortega S, de Torrontegui G, Díaz R

Abstract

The proteins P10 and P12 have been shown to be gene products of a new stability system, ParD, of plasmid R1. It is now shown that an R1 miniplasmid, pAB112, carrying a trans-complementable amber mutation in the gene of the P10 protein, is lethal for the host in the absence of suppression. This lethal effect is suppressed in a supF background and also by deletions in pAB112 that affect the gene of the P12 protein. These data indicate that the P12 protein has a lethal effect on the host and that this effect is neutralized by the P10 protein. The possibility that the stabilization conferred by the ParD system could be due to a counterselection, mediated by P12, of cells that lose the plasmid at cell division, is discussed.

MeSH Terms

Escherichia coli/genetics Genes, Bacterial Genes, Lethal Mutation Phenotype R Factors Suppression, Genetic

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Bravo A

Centro de Investigaciones Biológicas, (C.S.I.C.), Madrid, Spain.

Ortega S

de Torrontegui G

Díaz R

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00331316

Published

1988-12-00

Pages

146-51

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00331316

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Killing of Escherichia coli cells modulated by components of the stability system ParD of plasmid R1.

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