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PMID: 3071733 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The use of the luxA gene of the bacterial luciferase operon as a reporter gene.

Molecular & general genetics : MGG ·Vol. 215 ·No. 1 ·1988-12-00 ·Pages 1-9

Olsson O, Koncz C, Szalay AA

Abstract

Bacterial luciferase can be assayed rapidly and with high sensitivity both in vivo and in vitro. Here we demonstrate that the N-terminal hydrophobic domain of the alpha catalytic subunit of the luciferase enzyme is indispensable for enzyme activity, although N-terminal translational fusions with full luciferase activity can be obtained. Bacterial luciferase is therefore ideally suited as a reporter enzyme for gene fusion experiments. A list of vectors for the convenient use of the luciferase marker genes to monitor gene expression in vivo are presented.

MeSH Terms
Base Sequence Chromosome Deletion DNA, Bacterial/genetics Escherichia coli/genetics Genes, Bacterial Genetic Markers Genetic Vectors Luciferases/genetics Molecular Sequence Data Operon Plasmids Protein Biosynthesis Recombinant Fusion Proteins/genetics Transcription, Genetic
Chemicals
DNA, Bacterial Genetic Markers Recombinant Fusion Proteins Luciferases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Olsson O
Department of Plant Physiology, University of Umeå, Sweden.
Koncz C
Szalay A A
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28 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00331295
Published
1988-12-00
Pages
1-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00331295
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