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PMID: 3058298 Published · ppublish English Journal Article Review

Clinical laboratory applications of monoclonal antibodies.

Clinical microbiology reviews ·Vol. 1 ·No. 3 ·1988-07-00 ·Pages 313-29

Payne WJ, Marshall DL, Shockley RK, Martin WJ

Abstract

Monoclonal antibody (MAb) technology is well recognized as a significant development for producing specific serologic reagents to a wide variety of antigens in unlimited amounts. These reagents have provided the means for developing a number of highly specific and reproducible immunological assays for rapid and accurate diagnosis of an extensive list of diseases, including infectious diseases. The impact that MAbs have had in characterizing infectious disease pathogens, as well as their current and future applications for use in clinical microbiology laboratories, is reviewed. In addition, the advantages (and disadvantages) of the use of MAbs in a number of immunoassays, such as particle agglutination, radioimmunoassays, enzyme-linked immunosorbent assays, immunofluorescent-antibody assays, and immunohistology, are explored, including the use of these reagents in novel test system assays. Also, nucleic acid probe technology is compared with the use of MAbs from the perspective of their respective applications in the diagnosis of infectious disease agents. There is no question that hybridoma technology has the potential to alter significantly the methods currently used in most clinical microbiology laboratories.

MeSH Terms
Antibodies, Monoclonal Enzyme-Linked Immunosorbent Assay Humans Immunoassay Infections/diagnosis Nucleic Acid Probes
Chemicals
Antibodies, Monoclonal Nucleic Acid Probes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Payne W J
Murex Corporation, Norcross, Georgia 30071.
Marshall D L
Shockley R K
Martin W J
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  156. In vitro B-lymphocyte antigen priming against both non-immunogenic and immunogenic molecules requiring low amounts of antigen and applicable in hybridoma technology.
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  157. A simple new method for using antigens separated by polyacrylamide gel electrophoresis to stimulate lymphocytes in vitro after converting bands cut from Western blots into antigen-bearing particles.
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Article Info
Journal
Clinical microbiology reviews
Abbr.
Clin Microbiol Rev
ISSN
0893-8512
DOI
10.1128/CMR.1.3.313
Published
1988-07-00
Pages
313-29
Language
English
Region
United States
NLM ID
8807282
PMCID
PMC358053
Subset
IM
External Links
PubMed source DOI: 10.1128/CMR.1.3.313 PMC Full Text View journal details
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