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PMID: 3050986 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The B- to Z-DNA equilibrium in vivo is perturbed by biological processes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 19 ·1988-10-00 ·Pages 7069-73

Zacharias W, Jaworski A, Larson JE, Wells RD

Abstract

Right-handed B and left-handed Z conformations coexist in equilibrium in portions of plasmids in Escherichia coli. The equilibria are influenced by the length of the sequences that undergo the structural transitions and are perturbed by biological processes. The composite results of three types of determinations indicate a supercoil density of -0.025 in vivo. The coexistence of alternative DNA conformations in living cells implies the potential of these structures or their transitions for important functions in genetic regulatory processes.

MeSH Terms
DNA/metabolism DNA, Bacterial/metabolism Escherichia coli/genetics Gene Expression Regulation Nucleic Acid Conformation Plasmids Protein Synthesis Inhibitors/pharmacology Site-Specific DNA-Methyltransferase (Adenine-Specific)/metabolism
Chemicals
DNA, Bacterial Protein Synthesis Inhibitors DNA DNA modification methylase EcoRI Site-Specific DNA-Methyltransferase (Adenine-Specific)
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Zacharias W
University of Alabama School of Medicine, Department of Biochemistry, Birmingham 35294.
Jaworski A
Larson J E
Wells R D
References (26)
26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.19.7069
Published
1988-10-00
Pages
7069-73
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282125
Subset
IM
Grants
PHS HHS · 2 P60 20614-10 · United States
NIGMS NIH HHS · GM-30822 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.19.7069 PMC Full Text View journal details
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