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PMID: 3049624 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sequence of contactin, a 130-kD glycoprotein concentrated in areas of interneuronal contact, defines a new member of the immunoglobulin supergene family in the nervous system.

The Journal of cell biology ·Vol. 107 ·No. 4 ·1988-10-00 ·Pages 1561-73

Ranscht B

Abstract

The primary amino acid sequence of contactin, a neuronal cell surface glycoprotein of 130 kD that is isolated in association with components of the cytoskeleton (Ranscht, B., D. J. Moss, and C. Thomas. 1984. J. Cell Biol. 99:1803-1813), was deduced from the nucleotide sequence of cDNA clones and is reported here. The cDNA sequence contains an open reading frame for a 1,071-amino acid transmembrane protein with 962 extracellular and 89 cytoplasmic amino acids. In its extracellular portion, the polypeptide features six type 1 and two type 2 repeats. The six amino-terminal type 1 repeats (I-VI) each consist of 81-99 amino acids and contain two cysteine residues that are in the right context to form globular domains as described for molecules with immunoglobulin structure. Within the proposed globular region, contactin shares 31% identical amino acids with the neural cell adhesion molecule NCAM. The two type 2 repeats (I-II) are each composed of 100 amino acids and lack cysteine residues. They are 20-31% identical to fibronectin type III repeats. Both the structural similarity of contactin to molecules of the immunoglobulin supergene family, in particular the amino acid sequence resemblance to NCAM, and its relationship to fibronectin indicate that contactin could be involved in some aspect of cellular adhesion. This suggestion is further strengthened by its localization in neuropil containing axon fascicles and synapses.

MeSH Terms
Amino Acid Sequence Animals Antigens, Surface/genetics,physiology Base Sequence Blotting, Northern Brain/physiology Cell Adhesion Cell Adhesion Molecules Cell Adhesion Molecules, Neuronal Chick Embryo Cloning, Molecular Contactins DNA/genetics Fibronectins/genetics Immunoglobulins/genetics Immunologic Techniques Membrane Glycoproteins/genetics,physiology Molecular Sequence Data Molecular Structure Multigene Family Nerve Tissue Proteins/genetics,physiology Neurons/physiology
Chemicals
Antigens, Surface Cell Adhesion Molecules Cell Adhesion Molecules, Neuronal Contactins Fibronectins Immunoglobulins Membrane Glycoproteins Nerve Tissue Proteins DNA
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Ranscht B
Massachusetts Institute of Technology, Cambridge 02139.
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.107.4.1561
Published
1988-10-00
Pages
1561-73
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115254
Subset
IM
Grants
NINDS NIH HHS · NS 25194 · United States
Databases
GENBANK
Y00813
External Links
PubMed source DOI: 10.1083/jcb.107.4.1561 PMC Full Text View journal details
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