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PMID: 3046562 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Porcine parvovirus: replication in and inhibition of selected cellular functions of swine alveolar macrophages and peripheral blood lymphocytes.

Archives of virology ·Vol. 101 ·No. 1-2 ·1988-00-00 ·Pages 105-17

Harding MJ, Molitor TW

Abstract

The ability of four isolates of Porcine Parvovirus (NADL-8, NADL-2, KBSH, and Kresse) to replicate in and affect the functions of swine peripheral blood lymphocytes and alveolar macrophages was studied in vitro. V-strand and C-strand viral DNA was present in both concanavalin A- and non-treated lymphocytes as well as alveolar macrophages following infection with all four isolates. Indirect fluorescent antibody assays on swine testis cells, inoculated with cell lysates of NADL-8-infected peripheral blood lymphocytes (both concanavalin A- and non-treated) and alveolar macrophages, indicated that these immune cells supported the production of progeny virus. The quantity of viral DNA and progeny virus was dependent upon the multiplicity of infection and length of time following infection. Infection of lymphocytes and alveolar macrophages with PPV was associated with a decrease in cell viability. Peripheral blood mononuclear cells and alveolar macrophages infected with any of the four isolates demonstrated reduced lymphocyte blastogenesis and non-Fc-mediated alveolar macrophage phagocytosis, respectively.

MeSH Terms
Animals Cell Cycle Cell Survival Cells, Cultured Concanavalin A/pharmacology DNA, Viral/analysis,biosynthesis Fluorescent Antibody Technique Lymphocytes/analysis,cytology,microbiology Macrophages/analysis,microbiology Nucleic Acid Hybridization Parvoviridae/growth & development Phagosomes Swine Virion/analysis Virus Replication
Chemicals
DNA, Viral Concanavalin A
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Harding M J
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul.
Molitor T W
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Article Info
Journal
Archives of virology
Abbr.
Arch Virol
ISSN
0304-8608
DOI
10.1007/BF01314655
Published
1988-00-00
Pages
105-17
Language
English
Region
Austria
NLM ID
7506870
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01314655 View journal details
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