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PMID: 3041037 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Molecular genetic analysis of a vaccinia virus gene with an essential role in DNA replication.

Journal of virology ·Vol. 61 ·No. 10 ·1987-10-00 ·Pages 3152-62

Evans E, Traktman P

Abstract

We have identified a gene encoded by vaccinia virus which is essential for DNA replication. The gene, located in the HindIII D fragment of the viral genome, is transcribed early after infection into two transcripts of 3.0 and 3.7 kilobases which share a 3' terminus. The lesions of three temperature-sensitive DNA replication mutants with defects in this gene have been localized by marker rescue with progressively smaller DNA fragments. We have determined by hybrid selection that the gene encodes an 82-kilodalton protein. An antibody has been prepared against this polypeptide and used to quantitate expression of the protein after infection with wild-type virus or with a viral mutant whose lesion maps within this gene. The temporal pattern of expression in the mutant is unaffected, but the product encoded by the mutant is significantly more thermolabile than the wild-type protein.

MeSH Terms
Animals DNA Replication DNA Restriction Enzymes DNA, Viral/analysis Genes, Viral Genetic Markers Kinetics L Cells Mice Mutation Nucleic Acid Hybridization Plasmids Protein Biosynthesis RNA, Viral/analysis Transcription, Genetic Vaccinia virus/genetics,physiology Viral Proteins/genetics Virus Replication
Chemicals
DNA, Viral Genetic Markers RNA, Viral Viral Proteins DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Evans E
Traktman P
References (46)
46 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.10.3152-3162.1987
Published
1987-10-00
Pages
3152-62
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255892
Subset
IM
Grants
NIAID NIH HHS · R01 AI21758 · United States
External Links
PubMed source DOI: 10.1128/JVI.61.10.3152-3162.1987 PMC Full Text View journal details
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