Home LiteratureArticle Details
PMID: 3040694 Published · ppublish English Journal Article

Efficient transformation of phytopathogenic strains of Xanthomonas species.

Journal of bacteriology ·Vol. 169 ·No. 9 ·1987-09-00 ·Pages 4406-9

Murooka Y, Iwamoto H, Hamamoto A, Yamauchi T

Abstract

Efficient transformation and conjugation systems for use in phytopathogenic strains of Xanthomonas species were developed with a dual-function plasmid vector, pBXC12, which was constructed from a newly isolated Xanthomonas citri plasmid, pXCL6, and pBR328. By using this system, pBR328 could also transform the same strains of Xanthomonas. The systems make useful cloning vectors for the study of genes involved in the plant pathogenesis of this species.

MeSH Terms
Cloning, Molecular Conjugation, Genetic DNA Restriction Enzymes DNA, Bacterial/analysis Electrophoresis, Agar Gel Genes, Bacterial Genetic Vectors Nucleic Acid Hybridization Plants/microbiology Plasmids Transformation, Bacterial Xanthomonas/genetics
Chemicals
DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Murooka Y
Iwamoto H
Hamamoto A
Yamauchi T
References (7)
7 references, click to expand
  1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  2. Structure of extracellular polysaccharide from Xanthomonas campestris.
    Carbohydr Res. 1975 Dec;45:275-82 PMID: 1212669
  3. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  4. Trans-complementable copy-number mutants of plasmid ColE1.
    Nature. 1980 Jan 10;283(5743):216-8 PMID: 7350544
  5. Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325.
    Gene. 1980 May;9(3-4):287-305 PMID: 6248430
  6. Introduction of bacteriophage Mu into bacteria of various genera and intergeneric gene transfer by RP4::Mu.
    J Bacteriol. 1981 Jan;145(1):358-68 PMID: 6450749
  7. Studies on transformation of Escherichia coli with plasmids.
    J Mol Biol. 1983 Jun 5;166(4):557-80 PMID: 6345791
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
Published
1987-09-00
Pages
4406-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213764
Subset
IM
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com