Abstract
Efficient transformation and conjugation systems for use in phytopathogenic strains of Xanthomonas species were developed with a dual-function plasmid vector, pBXC12, which was constructed from a newly isolated Xanthomonas citri plasmid, pXCL6, and pBR328. By using this system, pBR328 could also transform the same strains of Xanthomonas. The systems make useful cloning vectors for the study of genes involved in the plant pathogenesis of this species.
MeSH Terms
Cloning, Molecular
Conjugation, Genetic
DNA Restriction Enzymes
DNA, Bacterial/analysis
Electrophoresis, Agar Gel
Genes, Bacterial
Genetic Vectors
Nucleic Acid Hybridization
Plants/microbiology
Plasmids
Transformation, Bacterial
Xanthomonas/genetics
Chemicals
DNA, Bacterial
DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Murooka Y
Iwamoto H
Hamamoto A
Yamauchi T
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7 references, click to expand
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