(2')3',5'-Bisphosphate nucleotidase.

The Journal of biological chemistry ·Vol. 262 ·No. 21 ·1987-07-25 ·Pages 10044-7

Ramaswamy SG, Jakoby WB

Abstract

(2')3',5'-Bisphosphate nucleotidase has been prepared in electrophoretically homogeneous form from guinea pig liver. The enzyme catalyzes the hydrolysis of the 2'- or 3'-phosphate from the appropriate nucleoside 2',5'- and 3',5'-bisphosphates and is active with 3'-phosphoadenosine 5'-phosphosulfate and with coenzyme A but not with ATP. The 40,000-dalton protein is a monomer that requires Mg2+ for activity.

MeSH Terms

Adenosine Triphosphate/metabolism Amino Acids/analysis Animals Chromatography, Affinity Chromatography, DEAE-Cellulose Coenzyme A/metabolism Guinea Pigs Hydrogen-Ion Concentration Kinetics Liver/enzymology Magnesium/metabolism Molecular Weight Nucleotidases/isolation & purification,metabolism Phosphoadenosine Phosphosulfate/metabolism

Chemicals

Amino Acids Phosphoadenosine Phosphosulfate Adenosine Triphosphate Nucleotidases bisphosphoadenylate 3'-nucleotidase Magnesium Coenzyme A

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Ramaswamy S G

Jakoby W B

Article Info

Journal

The Journal of biological chemistry

Abbr.

J Biol Chem

ISSN

0021-9258

Published

1987-07-25

Pages

10044-7

Language

English

Region

United States

NLM ID

2985121R

Subset

External Links

PubMed source View journal details

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(2')3',5'-Bisphosphate nucleotidase.

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