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PMID: 3037527 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Multiple sequence elements are required for regulation of human T-cell leukemia virus gene expression.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 14 ·1987-07-00 ·Pages 4919-23

Rosen CA, Park R, Sodroski JG, Haseltine WA

Abstract

The U3 region of the long terminal repeat (LTR) of human T-cell leukemia virus type I (HTLV-I) contains sequences that respond to the trans-activating transcription (tat) factor encoded by the pX region of the provirus. Results presented here show that there are multiple tat-responsive sequences within the LTR and that a single 21-nucleotide sequence, which is repeated three times within the U3 region, is sufficient to determine the response to the trans-activator. This sequence is capable of conferring a tat-responsive phenotype upon the HTLV-I and simian virus 40 promoters, independent of orientation. Sequences required for efficient HTLV-I LTR-directed gene expression are also located 3' to the site of RNA initiation, within the R and U5 regions of the LTR.

MeSH Terms
Base Sequence Deltaretrovirus/genetics Gene Expression Regulation Gene Products, tat Genes, Viral Oncogene Proteins, Viral/physiology Promoter Regions, Genetic Repetitive Sequences, Nucleic Acid Simian virus 40/genetics Transcription Factors/physiology
Chemicals
Gene Products, tat Oncogene Proteins, Viral Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rosen C A
Park R
Sodroski J G
Haseltine W A
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36 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.14.4919
Published
1987-07-00
Pages
4919-23
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC305218
Subset
IM
Grants
NCI NIH HHS · CA07580 · United States
NCI NIH HHS · CA36974 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.14.4919 PMC Full Text View journal details
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