Complete nucleotide sequence of the mouse lactate dehydrogenase-A functional gene: comparison of the exon-intron organization of dehydrogenase genes.

Genetics ·Vol. 116 ·No. 1 ·1987-05-00 ·Pages 99-105

Fukasawa KM, Li SS

Abstract

The complete sequence of 12,851 nucleotides of the mouse lactate dehydrogenase-A (LDH-A) gene has been determined. It includes eight exons, seven introns, promoter and regulatory regions. The B1 repetitive elements present in intron III and VI are oriented in opposite orientation, and they share 72% sequence homology. The exon-intron organization of mouse LDH-A gene is compared with the organizations of other dehydrogenase genes, and the molecular evolution of the nicotinamide adenine dinucleotide binding domains is discussed.

MeSH Terms

Animals Base Sequence DNA Restriction Enzymes Exons Genes Humans Introns L-Lactate Dehydrogenase/genetics Mice Mice, Inbred C57BL Promoter Regions, Genetic Rats Sequence Homology, Nucleic Acid Species Specificity

Chemicals

L-Lactate Dehydrogenase DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Fukasawa K M

Li S S

References (26)

26 references, click to expand

Evolution of a gene. Multiple genes for LDH isozymes provide a model of the evolution of gene structure, function and regulation.
Science. 1975 Jul 11;189(4197):102-14 PMID: 1138367
Molecular evolution of mammalian lactate dehydrogenase-A genes and pseudogenes: association of a mouse processed pseudogene with a B1 repetitive sequence.
Mol Biol Evol. 1986 Jul;3(4):330-42 PMID: 3444409
The nucleotide sequence of the ubiquitous repetitive DNA sequence B1 complementary to the most abundant class of mouse fold-back RNA.
Nucleic Acids Res. 1980 Mar 25;8(6):1201-15 PMID: 7433120
TEM-induced gene mutations at enzyme loci in the mouse.
Environ Mutagen. 1979;1(1):19-25 PMID: 162283
A system for shotgun DNA sequencing.
Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
The adult beta-globin genes of the "single" type mouse C57BL.
Cell. 1981 May;24(2):403-11 PMID: 7237554
Alcohol dehydrogenase gene of Drosophila melanogaster: relationship of intervening sequences to functional domains in the protein.
Proc Natl Acad Sci U S A. 1981 May;78(5):2717-21 PMID: 6789320
Cyclic AMP regulation of lactate dehydrogenase. Quantitation of lactate dehydrogenase M-subunit messenger RNA in isoproterenol-and N6,O2'-dibutyryl cyclic AMP-stimulated rat C6 glioma cells by hybridization analysis using a cloned cDNA probe.
J Biol Chem. 1981 Dec 10;256(23):12545-52 PMID: 6271788
A catalogue of splice junction sequences.
Nucleic Acids Res. 1982 Jan 22;10(2):459-72 PMID: 7063411
Evolutionary relationships of vertebrate lactate dehydrogenase isozymes A4 (muscle), B4 (heart), and C4 (testis).
J Biol Chem. 1983 Jun 10;258(11):7029-32 PMID: 6853510
Lactate dehydrogenase A-subunit and B-subunit deficiencies: comparison of the physiological roles of LDH isozymes.
Isozymes Curr Top Biol Med Res. 1983;7:131-50 PMID: 6411649
Molecular analysis of the alcohol dehydrogenase (Adh1) gene of maize.
Nucleic Acids Res. 1984 May 11;12(9):3983-4000 PMID: 6328449
Correlation of exons with structural domains in alcohol dehydrogenase.
EMBO J. 1984 Jun;3(6):1307-10 PMID: 6378620
Complete sequence of the chicken glyceraldehyde-3-phosphate dehydrogenase gene.
Proc Natl Acad Sci U S A. 1985 Mar;82(6):1628-32 PMID: 3856841
Protein structure and gene organization of mouse lactate dehydrogenase-A isozyme.
Eur J Biochem. 1985 Jun 3;149(2):215-25 PMID: 3996406
Epidermal growth factor or serum stimulation of rat fibroblasts induces an elevation in mRNA levels for lactate dehydrogenase and other glycolytic enzymes.
Nucleic Acids Res. 1985 Feb 11;13(3):711-26 PMID: 3873645
Isolation and characterization of a cDNA and a pseudogene for mouse lactate dehydrogenase-A isozyme.
Int J Biochem. 1985;17(5):645-8 PMID: 2993055
Structure of the human phosphoglycerate kinase gene and the intron-mediated evolution and dispersal of the nucleotide-binding domain.
Proc Natl Acad Sci U S A. 1985 Oct;82(20):6965-9 PMID: 2995995
Genomic organization of human lactate dehydrogenase-A gene.
Biochem J. 1985 Nov 1;231(3):537-41 PMID: 3000353
Molecular analysis of the human class I alcohol dehydrogenase gene family and nucleotide sequence of the gene encoding the beta subunit.
J Biol Chem. 1986 Feb 15;261(5):2027-33 PMID: 2935533
Characterization of the phosphoenolpyruvate carboxykinase (GTP) promoter-regulatory region. II. Identification of cAMP and glucocorticoid regulatory domains.
J Biol Chem. 1986 Jul 25;261(21):9721-6 PMID: 3015903
Primary structure of the mouse glycerol-3-phosphate dehydrogenase gene.
J Biol Chem. 1986 Sep 5;261(25):11779-85 PMID: 3755721
Identification of a cyclic-AMP-responsive element within the rat somatostatin gene.
Proc Natl Acad Sci U S A. 1986 Sep;83(18):6682-6 PMID: 2875459
A cyclic AMP- and phorbol ester-inducible DNA element.
Nature. 1986 Sep 25-Oct 1;323(6086):353-6 PMID: 3020428
Molecular nature of spontaneous mutations in mouse lactate dehydrogenase-A processed pseudogenes.
Genetics. 1987 Jan;115(1):177-84 PMID: 3557110
DNA sequencing with chain-terminating inhibitors.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968

Article Info

Journal

Genetics

Abbr.

Genetics

ISSN

0016-6731

DOI

10.1093/genetics/116.1.99

Published

1987-05-00

Pages

99-105

Language

English

Region

United States

NLM ID

0374636

PMCID

PMC1203126

Subset

External Links

PubMed source DOI: 10.1093/genetics/116.1.99 PMC Full Text View journal details

Analysis Services

Complete nucleotide sequence of the mouse lactate dehydrogenase-A functional gene: comparison of the exon-intron organization of dehydrogenase genes.

Contact

WeChat Official Account