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PMID: 3034609 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Yeast DNA polymerase--DNA primase complex; cloning of PRI 1, a single essential gene related to DNA primase activity.

The EMBO journal ·Vol. 6 ·No. 3 ·1987-03-00 ·Pages 737-42

Lucchini G, Francesconi S, Foiani M, Badaracco G, Plevani P

Abstract

The immunopurified yeast DNA polymerase--DNA primase complex is constituted by DNA polymerase I polypeptides and by three other protein species, called p74, p58 and p48, which we show to be immunologically unrelated. The gene encoding the p48 polypeptide has been identified by immunological screening of a lambda gt11 yeast genomic DNA library. Antiserum specific for p48 inhibits DNA primase, and immunoreactive, inhibitory antibodies are affinity-purified by the clone-encoded protein, thus relating the p48 polypeptide to DNA primase activity. The entire gene has been cloned, and the 1.45-kb p48 mRNA is overproduced in cells containing the gene in high copy number. Gene disruption and Southern hybridization experiments demonstrate that the p48 protein is encoded by a single gene and it performs an essential function.

MeSH Terms
Cloning, Molecular DNA Primase DNA Restriction Enzymes Escherichia coli/genetics Genes Genes, Fungal Nucleic Acid Hybridization Protein Biosynthesis RNA Nucleotidyltransferases/genetics,isolation & purification Saccharomyces cerevisiae/enzymology,genetics Transcription, Genetic
Chemicals
DNA Primase RNA Nucleotidyltransferases DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lucchini G
Francesconi S
Foiani M
Badaracco G
Plevani P
References (31)
31 references, click to expand
  1. Studies on deoxyribonucleic acid polymerases from yeast. 1. Parial purification and properties of two DNA polymerases from mitochondria-free cell extracts.
    Eur J Biochem. 1970 Mar 1;13(1):11-9 PMID: 4909093
  2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  3. Genetic control of the cell division cycle in yeast. II. Genes controlling DNA replication and its initiation.
    J Mol Biol. 1971 Jul 14;59(1):183-94 PMID: 5283752
  4. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  5. DNA polymerases from bakers' yeast.
    J Biol Chem. 1977 Mar 25;252(6):1873-80 PMID: 321447
  6. DNA rearrangements associated with a transposable element in yeast.
    Cell. 1980 Aug;21(1):239-49 PMID: 6250713
  7. Production of antisera with small doses of immunogen: multiple intradermal injections.
    Methods Enzymol. 1981;73(Pt B):46-52 PMID: 7300686
  8. Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase.
    Cell. 1982 Jan;28(1):145-54 PMID: 7039847
  9. A DNA primase activity associated with DNA polymerase alpha from Drosophila melanogaster embryos.
    Proc Natl Acad Sci U S A. 1982 Apr;79(8):2523-7 PMID: 6806812
  10. Lethal disruption of the yeast actin gene by integrative DNA transformation.
    Science. 1982 Jul 23;217(4557):371-3 PMID: 7046050
  11. Mouse DNA replicase. DNA polymerase associated with a novel RNA polymerase activity to synthesize initiator RNA of strict size.
    J Biol Chem. 1982 Sep 25;257(18):11121-7 PMID: 6179947
  12. DNA primase activity associated with DNA polymerase alpha from Xenopus laevis ovaries.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7209-13 PMID: 6961403
  13. Efficient isolation of genes by using antibody probes.
    Proc Natl Acad Sci U S A. 1983 Mar;80(5):1194-8 PMID: 6219389
  14. Repeated DNA sequences upstream from HIS1 also occur at several other co-regulated genes in Saccharomyces cerevisiae.
    J Biol Chem. 1983 Apr 25;258(8):5238-47 PMID: 6300123
  15. Isolation of an intact DNA polymerase-primase from embryos of Drosophila melanogaster.
    Proc Natl Acad Sci U S A. 1983 Apr;80(8):2221-5 PMID: 6403945
  16. A DNA primase from mouse cells. Purification and partial characterization.
    J Biol Chem. 1983 Aug 25;258(16):9845-9 PMID: 6885774
  17. Eviction and transplacement of mutant genes in yeast.
    Methods Enzymol. 1983;101:211-28 PMID: 6310325
  18. Yeast RNA polymerase II genes: isolation with antibody probes.
    Science. 1983 Nov 18;222(4625):778-82 PMID: 6356359
  19. DNA primase from KB cells. Characterization of a primase activity tightly associated with immunoaffinity-purified DNA polymerase-alpha.
    J Biol Chem. 1984 Feb 10;259(3):1854-65 PMID: 6693436
  20. Mouse primase initiation sites in the origin region of simian virus 40.
    Proc Natl Acad Sci U S A. 1984 Apr;81(8):2342-6 PMID: 6326122
  21. DNA polymerase I and DNA primase complex in yeast.
    J Biol Chem. 1984 Jun 25;259(12):7532-9 PMID: 6376490
  22. A DNA primase that copurifies with the major DNA polymerase from the yeast Saccharomyces cerevisiae.
    J Biol Chem. 1984 Jun 25;259(12):7936-40 PMID: 6376495
  23. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  24. Purification of a DNA polymerase-DNA primase complex from calf thymus glands.
    J Biol Chem. 1984 Dec 10;259(23):14679-87 PMID: 6094569
  25. Polypeptide structure of DNA primase from a yeast DNA polymerase-primase complex.
    J Biol Chem. 1985 Jun 10;260(11):7102-7 PMID: 3888995
  26. An electrophoretic karyotype for yeast.
    Proc Natl Acad Sci U S A. 1985 Jun;82(11):3756-60 PMID: 3889913
  27. Initiation, elongation and pausing of in vitro DNA synthesis catalyzed by immunopurified yeast DNA primase: DNA polymerase complex.
    EMBO J. 1985 May;4(5):1313-7 PMID: 4006921
  28. Isolation of the gene encoding yeast DNA polymerase I.
    Cell. 1985 Nov;43(1):369-77 PMID: 3907855
  29. Eukaryotic DNA replication.
    Annu Rev Biochem. 1986;55:733-71 PMID: 3017196
  30. Identification of the yeast DNA polymerase I gene with antibody probes.
    Curr Genet. 1985;10(4):245-52 PMID: 2842072
  31. Replacement of chromosome segments with altered DNA sequences constructed in vitro.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):4951-5 PMID: 388424
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-03-00
Pages
737-42
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553458
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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