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PMID: 3029420 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mutational analysis of open reading frame E4 of bovine papillomavirus type 1.

Journal of virology ·Vol. 61 ·No. 4 ·1987-04-00 ·Pages 1248-52

Neary K, Horwitz BH, DiMaio D

Abstract

Open reading frame (ORF) E4 is a 353-base-pair ORF of bovine papillomavirus type 1. To determine the biological activities of this ORF in mouse C127 cells, we analyzed the effects of two constructed mutations which are predicted to prevent synthesis of ORF E4 proteins while leaving the amino acid sequence encoded by the overlapping ORF E2 unchanged. Neither mutation interfered with the abilities of the mutants to efficiently induce focus formation, induce growth in soft agarose, or transactivate an inducible bovine papillomavirus type 1 enhancer. Also, neither mutation prevented establishment of the viral DNA as an extrachromosomal plasmid in transformed cells. These results suggest that ORF E4 proteins are not required for these biological activities, and they are consistent with the observation of others (J. Doorbar, D. Campbell, R. J. A. Grand, and P. H. Gallimore, EMBO J. 5:355-362, 1986) that the ORF E4 protein of a human papillomavirus is associated with late gene expression during papilloma formation.

MeSH Terms
Animals Bovine papillomavirus 1/genetics Cattle Cell Line Cell Transformation, Neoplastic DNA Restriction Enzymes Genes, Viral Mice Mutation Papillomaviridae/genetics Plasmids
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Neary K
Horwitz B H
DiMaio D
References (32)
32 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.4.1248-1252.1987
Published
1987-04-00
Pages
1248-52
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254088
Subset
IM
Grants
NCI NIH HHS · CA-37157 · United States
External Links
PubMed source DOI: 10.1128/JVI.61.4.1248-1252.1987 PMC Full Text View journal details
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