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PMID: 3029396 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Modification of RNA polymerase IIO subspecies after poliovirus infection.

Journal of virology ·Vol. 61 ·No. 4 ·1987-04-00 ·Pages 1002-6

Rangel LM, Fernandez-Tomas C, Dahmus ME, Gariglio P

Abstract

Infection of HeLa cells with poliovirus results in a shutdown of host transcription. In an effort to understand the mechanism(s) that underlies this process, we analyzed the distribution of RNA polymerase IIO before and after viral infection. Analysis of free and chromatin-bound enzyme indicated that there is a significant reduction in RNA polymerase IIO following infection. This observation, together with increasing evidence that transcription is catalyzed by RNA polymerase IIO, supports the hypothesis that poliovirus-induced inhibition of host transcription occurs at the level of RNA chain initiation and involves the direct modification of RNA polymerase II.

MeSH Terms
Cell Transformation, Viral Chromatin/enzymology HeLa Cells/enzymology Humans Kinetics Molecular Weight Poliovirus/enzymology,genetics RNA Polymerase II/genetics
Chemicals
Chromatin RNA Polymerase II
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rangel L M
Fernandez-Tomas C
Dahmus M E
Gariglio P
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32 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.4.1002-1006.1987
Published
1987-04-00
Pages
1002-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254056
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.61.4.1002-1006.1987 PMC Full Text View journal details
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