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PMID: 3023957 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nonhomologous recombination in the parvovirus chromosome: role for a CTATTTCT motif.

Molecular and cellular biology ·Vol. 6 ·No. 8 ·1986-08-00 ·Pages 3005-9

Hogan A, Faust EA

Abstract

The mechanism of nonhomologous recombination in murine cells infected with the parvovirus minute virus of mice (MVM) has been investigated by analysis of DNA sequences at recombination junctions in naturally occurring deletion variants of the virus. We report here that nonhomologous recombination in the MVM chromosome is characterized by short homologies, by insertion at recombination junctions of foreign DNA sequences that are enriched for preferred eucaryotic topoisomerase I cleavage sites, and by an association with a common DNA sequence motif of the type 5'-CTATTTCT-3'. Additional analyses of broken MVM chromosomes provided evidence for specific enzymatic cleavage within 5'-CTTATC-3' and 5'-CTATTC-3' sequences. The results indicate that the 5'-CTATTTCT-3' motif is an important genetic element for nonhomologous recombination in the parvovirus chromosome.

MeSH Terms
Animals Base Sequence Chromosome Deletion Chromosomes DNA Topoisomerases, Type I/metabolism DNA, Viral/analysis Mice Minute Virus of Mice/genetics Parvoviridae/genetics Recombination, Genetic
Chemicals
DNA, Viral DNA Topoisomerases, Type I
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hogan A
Faust E A
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27 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.8.3005-3009.1986
Published
1986-08-00
Pages
3005-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367873
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.6.8.3005-3009.1986 PMC Full Text View journal details
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