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PMID: 3023449 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Cyclic adenosine monophosphate-stimulated anion transport in rabbit cortical collecting duct. Kinetics, stoichiometry, and conductive pathways.

The Journal of clinical investigation ·Vol. 78 ·No. 6 ·1986-12-00 ·Pages 1621-30

Schuster VL

Abstract

Cyclic AMP stimulates HCO3 secretion and Cl self-exchange in rabbit cortical collecting tubule. We found that varying peritubular [Cl] changed the Cl self-exchange rate with saturation kinetics (Km, 3-4 mM). HCO3 secretion also showed saturation kinetics as a function of mean luminal [Cl] (Km, 4-11 mM). Both Cl self-exchange and Cl-HCO3 exchange thus appear to be carrier-mediated. Addition/removal of basolateral HCO3 qualitatively changed Cl and HCO3 transport as expected for Cl-HCO3 exchange, but quantitatively changed Cl absorption more than HCO3 secretion. The diffusive Cl permeability and the transepithelial conductance in the presence of HCO3/CO2 and cAMP were higher than in their absence suggesting that HCO3/CO2 and cAMP together increase a conductive Cl pathway parallel to a 1:1 Cl-HCO3 exchanger. Thus, cAMP not only stimulates the overall process of anion exchange (probably by increasing an electroneutral exchanger and/or a series Cl conductance), but also stimulates a Cl conductance parallel to the exchange process.

MeSH Terms
Absorption Animals Bicarbonates/metabolism Biological Transport/drug effects Chlorides/metabolism Cyclic AMP/pharmacology Kidney Cortex/metabolism Kidney Tubules/metabolism Kidney Tubules, Collecting/drug effects,metabolism Kinetics Permeability Rabbits
Chemicals
Bicarbonates Chlorides Cyclic AMP
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Schuster V L
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32 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI112755
Published
1986-12-00
Pages
1621-30
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC423935
Subset
IM
Grants
NIADDK NIH HHS · K08-AM01343 · United States
External Links
PubMed source DOI: 10.1172/JCI112755 PMC Full Text View journal details
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