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PMID: 3020555 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Development of the smooth muscle foam cell: uptake of macrophage lipid inclusions.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 20 ·1986-10-00 ·Pages 7760-4

Wolfbauer G, Glick JM, Minor LK, Rothblat GH

Abstract

A possible mechanism for the formation of smooth muscle foam cells in the atherosclerotic lesion was explored. Cultured macrophages (J774 cell line) were induced to form cytoplasmic cholesteryl ester inclusions by exposure to acetylated low density lipoprotein in the presence of cholesterol-rich phospholipid dispersions. The macrophages were disrupted by brief sonication, and the inclusions were isolated by flotation. When these inclusions were placed in direct contact with cultured smooth muscle cells, cellular uptake of the inclusions in a time- and dose-dependent manner was observed. Light and electron microscopy indicated the presence of lipid inclusions throughout the cytoplasm of the cells. Uptake of inclusion lipid by the smooth muscle cells was inhibited by several metabolic inhibitors, indicating that the process is dependent on metabolic activity. A modest but significant hydrolysis of the cholesteryl ester was observed, showing that the stored cholesteryl esters are metabolically available.

MeSH Terms
Arteriosclerosis/pathology Cells, Cultured Cholesterol Esters/metabolism Endocytosis Inclusion Bodies/metabolism Macrophages/metabolism Metabolic Clearance Rate Muscle, Smooth/metabolism,pathology Phagocytosis
Chemicals
Cholesterol Esters
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wolfbauer G
Glick J M
Minor L K
Rothblat G H
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39 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.20.7760
Published
1986-10-00
Pages
7760-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386801
Subset
IM
Grants
NHLBI NIH HHS · HL22633 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.20.7760 PMC Full Text View journal details
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