Home LiteratureArticle Details
PMID: 3020503 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Restriction endonucleases from Herpetosiphon giganteus: an example of the evolution of DNA recognition specificity?

Nucleic acids research ·Vol. 14 ·No. 17 ·1986-09-11 ·Pages 7031-45

Whitehead PR, Jacobs D, Brown NL

Abstract

We describe the partial purification and characterisation of five Type II restriction endonucleases from two strains of Herpetosiphon giganteus. One of the activities, HgiJII, was the first enzyme found that cleaves DNA at the family of related sequences 5'-G-R-G-C-Y/C-3'. This enzyme may be related to the enzyme HgiAI from a different strain of the same species, and which cleaves at the sites 5'-G-W-G-C-W/C-3'. We have shown that DNAs from the strains producing HgiAI and HgiJII are resistant to both of these restriction endonucleases. The remaining four enzymes described here share recognition and cleavage specificities with other restriction endonucleases. The evolution of Type II restriction-modification systems and their role in vivo are discussed.

MeSH Terms
Bacteroidetes/enzymology Biological Evolution DNA Restriction Enzymes/genetics,isolation & purification,metabolism DNA, Bacterial/genetics Substrate Specificity
Chemicals
DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Whitehead P R
Jacobs D
Brown N L
References (20)
20 references, click to expand
  1. Manipulation of restriction targets in phage lambda to form receptor chromosomes for DNA fragments.
    Nature. 1974 Oct 11;251(5475):476-81 PMID: 4608939
  2. The structure and function of the Eco RI restriction endonuclease.
    Gene Amplif Anal. 1981;1:131-64 PMID: 6765637
  3. The nucleotide sequence of bacteriophage phiX174.
    J Mol Biol. 1978 Oct 25;125(2):225-46 PMID: 731693
  4. Complete nucleotide sequence of the Escherichia coli plasmid pBR322.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:77-90 PMID: 383387
  5. Nucleotide sequence of small ColE1 derivatives: structure of the regions essential for autonomous replication and colicin E1 immunity.
    Mol Gen Genet. 1979 May 4;172(2):151-9 PMID: 384144
  6. A general method for defining restriction enzyme cleavage and recognition sites.
    Methods Enzymol. 1980;65(1):391-404 PMID: 6246360
  7. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  8. HgiAI: a restriction endonuclease from Herpetosiphon giganteus HP1023.
    Gene. 1980 Apr;9(1-2):49-68 PMID: 6247247
  9. Identification of palindromic sequences recognized by restriction endonucleases, as based on the tabularized sequencing data for seven viral and plasmid DNAs.
    Gene. 1980 Sep;10(4):357-70 PMID: 6253357
  10. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  11. AhaIII: a restriction endonuclease with a recognition sequence containing only A:T basepairs.
    FEBS Lett. 1982 Jul 5;143(2):296-300 PMID: 6288466
  12. EaeI: a restriction endonuclease from Enterobacter aerogenes.
    FEBS Lett. 1983 May 2;155(1):97-101 PMID: 6301886
  13. Bacteriophage survival: multiple mechanisms for avoiding the deoxyribonucleic acid restriction systems of their hosts.
    Microbiol Rev. 1983 Sep;47(3):345-60 PMID: 6314109
  14. Eight new restriction endonucleases fröm Herpetosiphon giganteus--divergent evolution in a family of enzymes.
    Nucleic Acids Res. 1984 Apr 11;12(7):3127-41 PMID: 6326052
  15. Mercuric ion-resistance operons of plasmid R100 and transposon Tn501: the beginning of the operon including the regulatory region and the first two structural genes.
    Proc Natl Acad Sci U S A. 1984 Oct;81(19):5975-9 PMID: 6091128
  16. Three restriction endonucleases from Anabaena flos-aquae.
    J Gen Microbiol. 1985 Apr;131(4):951-8 PMID: 2985742
  17. Restriction and modification enzymes and their recognition sequences.
    Nucleic Acids Res. 1985;13 Suppl:r165-200 PMID: 2987885
  18. The effect of site specific methylation on restriction endonuclease digestion.
    Nucleic Acids Res. 1985;13 Suppl:r201-7 PMID: 2987886
  19. A comparison of the structural requirements for DNA cleavage by the isoschizomers HaeIII, BspRI and BsuRI.
    Eur J Biochem. 1985 Jul 1;150(1):105-10 PMID: 2990922
  20. Analysis of bacteriophage deoxyribonucleic acid sequences methylated by host- and R-factor-controlled enzymes.
    J Bacteriol. 1975 Aug;123(2):768-70 PMID: 1097428
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.17.7031
Published
1986-09-11
Pages
7031-45
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311715
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/14.17.7031 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2