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PMID: 3020380 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Iron hydroxamate transport of Escherichia coli: nucleotide sequence of the fhuB gene and identification of the protein.

Molecular & general genetics : MGG ·Vol. 204 ·No. 3 ·1986-09-00 ·Pages 435-42

Köster W, Braun V

Abstract

The genes fhuA, fhuC and fhuD encode products involved in the transport of ferrichrome into cells of Escherichia coli. The nucleotide sequence of an additional locus contiguous to fhuD and formerly designated fhuB was determined. It contains an open reading frame for a polypeptide that consists of 659 amino acids. Expression of plasmids containing the fhuB region in an in vitro transcription/translation system and in E. coli minicells revealed a polypeptide with an electrophoretic mobility on SDS-polyacrylamide gels extremely dependent on the experimental conditions employed. This property could be explained by the very hydrophobic nature of the protein and it was concluded that the fhuB locus consists of a structural gene that encodes a membrane protein with a molecular weight of 82,181. The fhuB gene is required for all iron transport systems which operate via hydroxamate compounds. The order of the genes in the fhu operon is fhuA fhuC fhuD fhuB, and transcription proceeds from fhuA to fhuB.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence DNA Restriction Enzymes Escherichia coli/genetics,metabolism Genes Genes, Bacterial Genotype Hydroxamic Acids/metabolism Iron/metabolism Mutation
Chemicals
Bacterial Proteins Hydroxamic Acids Iron DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Köster W
Braun V
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00331021
Published
1986-09-00
Pages
435-42
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
X04319
External Links
PubMed source DOI: 10.1007/BF00331021
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