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PMID: 3019665 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Full-length von Willebrand factor (vWF) cDNA encodes a highly repetitive protein considerably larger than the mature vWF subunit.

The EMBO journal ·Vol. 5 ·No. 8 ·1986-08-00 ·Pages 1839-47

Verweij CL, Diergaarde PJ, Hart M, Pannekoek H

Abstract

Full-length human von Willebrand factor (vWF) cDNA was assembled from partial, overlapping vWF cDNAs. This cDNA construct includes a coding sequence of 8439 nucleotides which encode a single-chain precursor of 2813 amino-acid residues, representing a putative signal peptide, a prosequence and mature vWF of 22, 741 and 2050 amino acids, respectively. This represents the longest coding sequence determined to date. In-vitro expression of full-length vWF cDNA revealed the synthesis of a polypeptide with a mol. wt corresponding with that of the unglycosylated precursor. The precursor is a highly repetitive protein which consists of two duplicated (B, C), a triplicated (A), a quadruplicated (D) and a partly duplicated domain (D'), in the following order: H-D1-D2-D'-D3-A1-A2-A3-D4-B1-B2-C1-C2-OH. Both the prosequence, composed of two D domains (D1, D2), and mature vWF harbor an arg-gly-asp ('R-G-D') sequence which has been implicated in cell-attachment functions. It is argued that the pro-sequence is equivalent to von Willebrand Antigen II (vW AgII).

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA/metabolism DNA Restriction Enzymes Endonucleases Humans Macromolecular Substances Molecular Weight Plasmids RNA, Messenger/genetics Repetitive Sequences, Nucleic Acid Single-Strand Specific DNA and RNA Endonucleases von Willebrand Factor/genetics
Chemicals
Macromolecular Substances RNA, Messenger von Willebrand Factor DNA Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Verweij C L
Diergaarde P J
Hart M
Pannekoek H
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40 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-08-00
Pages
1839-47
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1167049
Subset
IM
Databases
GENBANK
X04146
Corrections
ErratumIn
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External Links
PubMed source PMC Full Text View journal details
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