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PMID: 3018287 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Molecular analysis and pathogenesis of the feline aplastic anemia retrovirus, feline leukemia virus C-Sarma.

Journal of virology ·Vol. 60 ·No. 1 ·1986-10-00 ·Pages 242-50

Riedel N, Hoover EA, Gasper PW, Nicolson MO, Mullins JI

Abstract

We describe the molecular cloning of an anemogenic feline leukemia virus (FeLV), FeLV-C-Sarma, from the productively infected human rhabdomyosarcoma cell line RD(FeLV-C-S). Molecularly cloned FeLV-C-S proviral DNA yielded infectious virus (mcFeLV-C-S) after transfection of mammalian cells, and virus interference studies using transfection-derived virus demonstrated that our clone encodes FeLV belonging to the C subgroup. mcFeLV-C-S did not induce viremia in eight 8-week-old outbred specific-pathogen-free (SPF) cats. It did, however, induce viremia and a rapid, fatal aplastic anemia due to profound suppression of erythroid stem cell growth in 9 of 10 inoculated newborn, SPF cats within 3 to 8 weeks (21 to 58 days) postinoculation. Thus, the genome of mcFeLV-C-S encodes the determinants responsible for the genetically dominant induction of irreversible erythroid aplasia in outbred cats. A potential clue to the pathogenic determinants of this virus comes from previous work indicating that all FeLV isolates belonging to the C subgroup, an envelop-gene-determined property, and only those belonging to the C subgroup, are potent, consistent inducers of aplastic anemia in cats. To approach the molecular mechanism underlying the induction of this disease, we first determined the nucleotide sequence of the envelope genes and 3' long terminal repeat of FeLV-C-S and compared it with that of FeLV-B-Gardner-Arnstein (mcFeLV-B-GA), a subgroup-B feline leukemia virus that consistently induces a different disease, myelodysplastic anemia, in neonatal SPF cats. Our analysis revealed that the p15E genes and long terminal repeats of the two FeLV strains are highly homologous, whereas there are major differences in the gp70 proteins, including five regions of significant amino acid differences and apparent sequence substitution. Some of these changes are also reflected in predicted glycosylation sites; the gp70 protein of FeLV-B-GA has 11 potential glycosylation sites, only 8 of which are present in FeLV-C-S.

MeSH Terms
Anemia, Aplastic/etiology,metabolism,veterinary Animals Base Sequence Cat Diseases/etiology,metabolism Cats Genes, Viral Leukemia Virus, Feline/genetics,isolation & purification,pathogenicity Repetitive Sequences, Nucleic Acid Retroviridae Proteins/genetics Viral Envelope Proteins/genetics Viremia/etiology,veterinary Virus Cultivation
Chemicals
Retroviridae Proteins Viral Envelope Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Riedel N
Hoover E A
Gasper P W
Nicolson M O
Mullins J I
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.60.1.242-250.1986
Published
1986-10-00
Pages
242-50
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253922
Subset
IM
Grants
NCI NIH HHS · CA32563 · United States
Databases
GENBANK
M14331
External Links
PubMed source DOI: 10.1128/JVI.60.1.242-250.1986 PMC Full Text View journal details
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