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PMID: 3015004 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Leakage of periplasmic proteins from Escherichia coli mediated by polymyxin B nonapeptide.

Antimicrobial agents and chemotherapy ·Vol. 29 ·No. 5 ·1986-05-00 ·Pages 781-8

Dixon RA, Chopra I

Abstract

The effects of polymyxin B and polymyxin B nonapeptide (PMBN) on cell envelope integrity in Escherichia coli were compared. Both compounds caused loss of proteins from E. coli K-12 3300(pBR322), although PMBN released less protein than did polymyxin B. The origin of the released protein was determined both by polyacrylamide gel electrophoresis and by using specific enzyme markers (beta-lactamase in periplasm, beta-galactosidase in cytoplasm). The proteins released by both compounds were derived principally from the periplasm, accompanied in the case of polymyxin B by a low level of cytoplasmic proteins. Although polymyxin B and PMBN both caused release of periplasmic proteins, the individual proteins released by the compounds differed. The periplasmic fraction contained six principal polypeptides with molecular weights between 62,000 (polypeptide 1) and 29,000 (polypeptide 6). Polypeptide 6 was identified as the pBR322-encoded beta-lactamase, but the other proteins were not specifically identified. Polymyxin B caused considerable release of polypeptides 1, 2, and 5 with some release of polypeptides 4 and 6. PMBN released polypeptide 1 (trace), 3, 4, and 6 (trace). Scanning electron microscopy showed that polymyxin B and PMBN both caused surface damage in E. coli. However, polymyxin B produced greater morphological changes than PMBN.

MeSH Terms
Bacterial Proteins/metabolism Electrophoresis, Polyacrylamide Gel Escherichia coli/drug effects,metabolism,ultrastructure Fusidic Acid/metabolism Kinetics Microscopy, Electron, Scanning Polymyxin B/analogs & derivatives,pharmacology Polymyxins/pharmacology
Chemicals
Bacterial Proteins Polymyxins Fusidic Acid polymyxin B nonapeptide Polymyxin B
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dixon R A
Chopra I
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33 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.29.5.781
Published
1986-05-00
Pages
781-8
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC284154
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.29.5.781 PMC Full Text View journal details
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