Osteonectin mRNA: distribution in normal and transformed cells.

Nucleic acids research ·Vol. 14 ·No. 11 ·1986-06-11 ·Pages 4483-97

Young MF, Bolander ME, Day AA, Ramis CI, Robey PG, Yamada Y, Termine JD

Abstract

Overlapping cDNA clones encoding bovine osteonectin were isolated from a lambda gt11 expression library constructed from bovine bone cell mRNA. The longest clone, lambda On 17 (insert size 2.0 kb) was studied in detail. The clone was shown to encode osteonectin by hybrid select translation experiments and by DNA sequence analysis. Northern analysis of bone cell RNA showed the length of the osteonectin mRNA to be 2.0 kb. Osteonectin message was found in bone but not in soft tissue (liver and brain) preparations consistent with the distribution of the protein in these tissues. On the other hand, osteonectin message was observed in tendon, a tissue in which little or no osteonectin protein is found in vivo. Hybridization of osteonectin cDNA was detected in cells from a number of species including human, rat, mouse and chick. The level of osteonectin mRNA was drastically decreased in chick embryo fibroblasts transformed by Rous sarcoma virus.

MeSH Terms

Animals Carrier Proteins/genetics Cattle Cell Transformation, Viral Cells, Cultured Chickens Chromosome Mapping Cloning, Molecular DNA/genetics DNA Restriction Enzymes Gene Expression Regulation Humans Molecular Weight Osteonectin RNA, Messenger/genetics Rats Sequence Homology, Nucleic Acid Species Specificity Tissue Distribution

Chemicals

Carrier Proteins Osteonectin RNA, Messenger DNA DNA Restriction Enzymes

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Young M F

Bolander M E

Day A A

Ramis C I

Robey P G

Yamada Y

Termine J D

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/14.11.4483

Published

1986-06-11

Pages

4483-97

Language

English

Region

England

NLM ID

0411011

PMCID

PMC311460

Subset

External Links

PubMed source DOI: 10.1093/nar/14.11.4483 PMC Full Text View journal details

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Osteonectin mRNA: distribution in normal and transformed cells.

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