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PMID: 3010949 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Insertion of transposon Tn7 into the Escherichia coli glmS transcriptional terminator.

The Biochemical journal ·Vol. 234 ·No. 1 ·1986-02-15 ·Pages 111-7

Gay NJ, Tybulewicz VL, Walker JE

Abstract

The transposon Tn7 is unusual as it transposes at high frequencies from episomal elements to a unique site in the Escherichia coli chromosome. This unique site is within a region of dyad symmetry that we have demonstrated to be the transcriptional terminator of the glmS gene which encodes the glutamine amidotransferase, glucosamine synthetase. Transposition of Tn7 abolishes termination of glmS transcription at this site; the transcripts now extend into the left end of Tn7 and terminate at a new site, tm, 127 base pairs from the left end of Tn7. This region of the transposon contains a long open reading frame which encodes a protein sequence that is significantly related to the transposase proteins of the transposable elements IS1 and Tn3. A weak transcript has been identified that emanates from a promoter on the 5' side of this reading frame. This promoter is over-run by glmS transcripts and so it appears that expression of the Tn7 transposase may be regulated by promoter occlusion.

MeSH Terms
Base Sequence Binding Sites DNA Transposable Elements DNA, Bacterial/genetics Electrophoresis, Polyacrylamide Gel Escherichia coli/genetics Gene Expression Regulation Genes, Regulator Nucleotidyltransferases/genetics Terminator Regions, Genetic Transcription, Genetic Transposases
Chemicals
DNA Transposable Elements DNA, Bacterial Nucleotidyltransferases Transposases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gay N J
Tybulewicz V L
Walker J E
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24 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2340111
Published
1986-02-15
Pages
111-7
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1146532
Subset
IM
Databases
GENBANK
M24254, M24255
External Links
PubMed source DOI: 10.1042/bj2340111 PMC Full Text View journal details
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