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PMID: 30101373 Published · ppublish English Journal Article

Fucosylation genes as circulating biomarkers for lung cancer.

Journal of cancer research and clinical oncology ·Vol. 144 ·No. 11 ·2018-11-00 ·Pages 2109-2115

Leng Q, Tsou JH, Zhan M, Jiang F

Abstract

Fucosyltransferases (FUTs) catalyze fucosylation, which plays a central role in biological processes. Aberrant fucosylation is associated with malignant transformation. Here we investigated whether transcriptional levels of genes coding the FUTs in plasma could provide cell-free circulating biomarkers for lung cancer. mRNA expression of all 13 Futs (Fut1-11, Pofut1, and Pofut2) was evaluated by PCR assay in 48 lung tumor tissues and the 48 matched noncancerous lung tissues, and plasma of 64 lung cancer patients and 32 cancer-free individuals to develop plasma Fut biomarkers. The developed plasma Fut biomarkers were validated in an independent cohort of 40 lung cancer patients and 20 controls for their diagnostic performance. Four of the 13 Futs showed a different transcriptional level in 48 lung tumor tissues compared with the 48 matched nonconscious tissues (all < 0.05). Two (Fut8, and Pofut1) of the four Futs had a higher plasma level in 64 lung cancer patients compared with 32 control subjects, and consistent with that in lung tissue specimens. Combined analysis of the two Futs produced 81% sensitivity and 86% specificity for diagnosis of lung cancer, and was independent of stage and histology of lung tumors. The diagnostic performance of the two plasma biomarkers was successfully validated in the different cohort of 40 lung cancer patients and 20 control individuals. The fucosylation genes may provide new circulating biomarkers for the early detection of lung cancer.

Keywords
Biomarkers Diagnosis Fucosyltransferases Lung cancer Plasma
MeSH Terms
Aged Biomarkers, Tumor/blood,genetics Female Fucose/metabolism Fucosyltransferases/genetics Gene Expression Regulation, Neoplastic Glycosylation Humans Isoenzymes/genetics Lung Neoplasms/blood,diagnosis,genetics Male Middle Aged Multigene Family RNA, Messenger/blood,genetics ROC Curve
Chemicals
Biomarkers, Tumor Isoenzymes RNA, Messenger Fucose Fucosyltransferases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Leng Qixin
Department of Pathology, The University of Maryland School of Medicine, 10 South Pine Street, MSTF 7th Floor, Baltimore, MD, 21201-1192, USA.
Tsou Jen-Hui
Department of Pathology, The University of Maryland School of Medicine, 10 South Pine Street, MSTF 7th Floor, Baltimore, MD, 21201-1192, USA.
Zhan Min
Department of Epidemiology and Public Health, University of Maryland School of Medicine, 660 W. Redwood St., Baltimore, MD, 21201, USA.
Jiang Feng ORCID
Department of Pathology, The University of Maryland School of Medicine, 10 South Pine Street, MSTF 7th Floor, Baltimore, MD, 21201-1192, USA. fjiang@som.umaryland.edu.
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Article Info
Journal
Journal of cancer research and clinical oncology
Abbr.
J Cancer Res Clin Oncol
ISSN
1432-1335
DOI
10.1007/s00432-018-2735-0
Email
fjiang@som.umaryland.edu
Published
2018-11-00
Epub
2018-00-12
Pages
2109-2115
Language
English
Region
Germany
NLM ID
7902060
Subset
IM
Grants
National Cancer Institute · R21CA205746
External Links
PubMed source DOI: 10.1007/s00432-018-2735-0 View journal details
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