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PMID: 3007110 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A new site of integration for mouse mammary tumor virus proviral DNA common to BALB/cf(C3H) mammary and kidney adenocarcinomas.

The EMBO journal ·Vol. 5 ·No. 1 ·1986-01-00 ·Pages 127-34

Garcia M, Wellinger R, Vessaz A, Diggelmann H

Abstract

The BALB/cf/Cd substrain of mice, developed by inbreeding and selection, shows a 70% incidence of spontaneous kidney adenocarcinomas. Initially foster-nursed on C3H mothers, these mice no longer produce mammary tumors, but there is evidence that mouse mammary tumor virus (MMTV) is involved in the formation of these renal carcinomas. We identified a chromosomal region called int-41, representing a locus interrupted by the integration of an exogenous MMTV provirus in a BALB/c mammary tumor. We found a DNA rearrangement and transcriptional activation in the domain specified by the int-41 probe in one primary kidney adenocarcinoma. A 5.2-kb int-41 specific mRNA was detected in the kidney tumor cell line established from a transplantable renal adenocarcinoma. This mRNA hybridized with MMTV and int-41 specific probes suggesting that it is a hybrid molecule composed of MMTV-LTR sequences covalently linked to host cell RNA. This mRNA was strongly stimulated by the presence of glucocorticoid hormone in the culture medium. Our data are compatible with the hypothesis that the int-41 chromosomal domain is involved in the neoplastic transformation of epithelial cells from different organs.

MeSH Terms
Adenocarcinoma/genetics,microbiology Animals Cell Line Chimera Cloning, Molecular DNA Restriction Enzymes DNA, Neoplasm/genetics DNA, Viral/genetics Kidney Neoplasms/genetics,microbiology Mammary Neoplasms, Experimental/genetics,microbiology Mammary Tumor Virus, Mouse/genetics Mice Mice, Inbred Strains Nucleic Acid Hybridization Protein Biosynthesis Transcription, Genetic
Chemicals
DNA, Neoplasm DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Garcia M
Wellinger R
Vessaz A
Diggelmann H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-01-00
Pages
127-34
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1166704
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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