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PMID: 3005656 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Pathogenicity in mice of herpes simplex virus type 2 mutants unable to express glycoprotein C.

Journal of virology ·Vol. 58 ·No. 1 ·1986-04-00 ·Pages 36-42

Johnson DC, McDermott MR, Chrisp C, Glorioso JC

Abstract

Herpes simplex virus type 2 (HSV-2) mutants that were unable to express glycoprotein C (gC-2) were isolated. Deletions were made in a cloned copy of the gC-2 gene, and recombinant viruses containing these deletions were screened by using an immunoreactive plaque selection protocol. The viruses did not display a syncytial phenotype. Intravaginal inoculation of BALB/cJ mice with one of the HSV-2 gC-2- viruses produced local inflammation followed by a lethal spread of the viral infection into the nervous system in a manner identical to that produced by parental HSV-2 strain 333. Similarly, intracerebral inoculation of DBA-2 mice with the gC-2- virus produced a lethal neurological disease paralleling that caused by HSV-2 strain 333. These results indicate that gC-2 is not required for the spread of HSV-2 infections in mice.

MeSH Terms
Animals Brain Diseases/microbiology DNA, Recombinant Female Herpes Simplex/microbiology Mice Mutation Simplexvirus/genetics,pathogenicity Transfection Vaginal Diseases/microbiology Viral Envelope Proteins/genetics,metabolism Virus Replication
Chemicals
DNA, Recombinant Viral Envelope Proteins glycoprotein C, herpes simplex virus type 2 glycoprotein gC, herpes simplex virus type 1
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Johnson D C
McDermott M R
Chrisp C
Glorioso J C
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48 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.58.1.36-42.1986
Published
1986-04-00
Pages
36-42
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC252873
Subset
IM
Grants
NCRR NIH HHS · RR00200-2 · United States
External Links
PubMed source DOI: 10.1128/JVI.58.1.36-42.1986 PMC Full Text View journal details
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