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PMID: 3002730 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

High resolution mapping of in situ hybridized biotinylated DNA to surface-spread Drosophila polytene chromosomes.

Chromosoma ·Vol. 93 ·No. 2 ·1985-00-00 ·Pages 113-22

Kress H, Meyerowitz EM, Davidson N

Abstract

We describe a method of mapping genes or transcripts on polytene chromosomes by transmission electron microscopy. We present several applications which illustrate that, in favorable cases, the method has a resolution of ca. 10 kg, and that high resolution mapping of hybridization sites relative to bands and puffs can be achieved. We mapped sites of transcription for poly-(A) RNA and present evidence which shows that these sites are localized in some bands and puffs, but are also found in interbands.

MeSH Terms
Animals Autoradiography Biotin Chromosome Mapping DNA/genetics DNA Restriction Enzymes Drosophila melanogaster/genetics Microscopy, Electron Nucleic Acid Hybridization Tritium
Chemicals
Tritium Biotin DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kress H
Meyerowitz E M
Davidson N
References (31)
31 references, click to expand
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00293158
Published
1985-00-00
Pages
113-22
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00293158 View journal details
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