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PMID: 3001642 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Analysis of cloned cDNA and genomic sequences for phytochrome: complete amino acid sequences for two gene products expressed in etiolated Avena.

Nucleic acids research ·Vol. 13 ·No. 23 ·1985-12-09 ·Pages 8543-59

Hershey HP, Barker RF, Idler KB, Lissemore JL, Quail PH

Abstract

Cloned cDNA and genomic sequences have been analyzed to deduce the amino acid sequence of phytochrome from etiolated Avena. Restriction endonuclease site polymorphism between clones indicates that at least four phytochrome genes are expressed in this tissue. Sequence analysis of two complete and one partial coding region shows approximately 98% homology at both the nucleotide and amino acid levels, with the majority of amino acid changes being conservative. High sequence homology is also found in the 5'-untranslated region but significant divergence occurs in the 3'-untranslated region. The phytochrome polypeptides are 1128 amino acid residues long corresponding to a molecular mass of 125 kdaltons. The known protein sequence at the chromophore attachment site occurs only once in the polypeptide, establishing that phytochrome has a single chromophore per monomer covalently linked to Cys-321. Computer analyses of the amino acid sequences have provided predictions regarding a number of structural features of the phytochrome molecule.

MeSH Terms
Amino Acid Sequence Base Sequence Chromosome Mapping Cloning, Molecular DNA/genetics DNA Restriction Enzymes/metabolism Edible Grain/genetics Gene Expression Regulation/radiation effects Genes Light Nucleic Acid Conformation Phytochrome/genetics Plant Proteins/genetics Sequence Homology, Nucleic Acid Solubility
Chemicals
Plant Proteins Phytochrome DNA DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hershey H P
Barker R F
Idler K B
Lissemore J L
Quail P H
References (29)
29 references, click to expand
  1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  2. Light-stimulated transcription of genes for two chloroplast polypeptides in isolated pea leaf nuclei.
    EMBO J. 1982;1(12):1493-8 PMID: 16453439
  3. On the use of sequence homologies to predict protein structure: identical pentapeptides can have completely different conformations.
    Proc Natl Acad Sci U S A. 1984 Feb;81(4):1075-8 PMID: 6422466
  4. Tetranitromethane oxidation of phytochrome chromophore as a function of spectral form and molecular weight.
    Plant Physiol. 1984 Apr;74(4):755-8 PMID: 16663505
  5. Use of a recombination-deficient phage lambda system to construct wheat genomic libraries.
    Gene. 1984 Oct;30(1-3):237-40 PMID: 6096216
  6. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  7. Phytochrome Control of the Expression of Two Nuclear Genes Encoding Chloroplast Proteins in Lemna gibba L. G-3.
    Plant Physiol. 1983 Jul;72(3):717-24 PMID: 16663073
  8. Autoregulatory control of translatable phytochrome mRNA levels.
    Proc Natl Acad Sci U S A. 1983 Apr;80(8):2248-52 PMID: 16578769
  9. An inverse control by phytochrome of the expression of two nuclear genes in barley (Hordeum vulgare L.).
    Eur J Biochem. 1984 Sep 17;143(3):593-7 PMID: 6383824
  10. Protozoan and related photoreceptors: molecular aspects.
    Annu Rev Biophys Bioeng. 1983;12:35-68 PMID: 6408980
  11. Demonstration of transcriptional regulation of specific genes by phytochrome action.
    Proc Natl Acad Sci U S A. 1984 Feb;81(4):1112-6 PMID: 16593420
  12. Rapid and sensitive protein similarity searches.
    Science. 1985 Mar 22;227(4693):1435-41 PMID: 2983426
  13. Plasmid screening at high colony density.
    Methods Enzymol. 1983;100:333-42 PMID: 6194407
  14. Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.
    J Mol Biol. 1978 Mar 25;120(1):97-120 PMID: 642007
  15. Monoclonal antibodies to three separate domains on 124 kilodalton phytochrome from Avena.
    Plant Physiol. 1984 Nov;76(3):622-6 PMID: 16663895
  16. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  17. Structure function studies on phytochrome. Identification of light-induced conformational changes in 124-kDa Avena phytochrome in vitro.
    J Biol Chem. 1985 Feb 25;260(4):2415-23 PMID: 3882693
  18. Isolation and partial characterization of a chromophore-peptide fragment from pepsin digests of phytochrome.
    Biochem Biophys Res Commun. 1971 Dec 17;45(6):1466-73 PMID: 4942730
  19. Partial characterization of undegraded oat phytochrome.
    Biochemistry. 1980 Jan 22;19(2):390-4 PMID: 7352993
  20. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  21. Native phytochrome: Inhibition of proteolysis yields a homogeneous monomer of 124 kilodaltons from Avena.
    Proc Natl Acad Sci U S A. 1982 Sep;79(17):5272-6 PMID: 16593222
  22. Control by Phytochrome of Cytoplasmic Precursor rRNA Synthesis in the Cotyledons of Mustard Seedlings.
    Plant Physiol. 1982 May;69(5):1156-60 PMID: 16662362
  23. Empirical predictions of protein conformation.
    Annu Rev Biochem. 1978;47:251-76 PMID: 354496
  24. Molecular cloning of cDNA for Avena phytochrome.
    Proc Natl Acad Sci U S A. 1984 Apr;81(8):2332-6 PMID: 16593453
  25. A computer program for predicting protein antigenic determinants.
    Mol Immunol. 1983 Apr;20(4):483-9 PMID: 6191210
  26. Different Red Light Requirements for Phytochrome-Induced Accumulation of cab RNA and rbcS RNA.
    Science. 1984 Dec 21;226(4681):1447-9 PMID: 17789001
  27. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  28. Phytochrome control of in vitro transcription of specific genes in isolated nuclei from barley (Hordeum vulgare).
    Eur J Biochem. 1985 Feb 15;147(1):137-42 PMID: 3882421
  29. Regulated expression of three alcohol dehydrogenase genes in barley aleurone layers.
    Plant Physiol. 1984 Jul;75(3):573-81 PMID: 16663668
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.23.8543
Published
1985-12-09
Pages
8543-59
Language
English
Region
England
NLM ID
0411011
PMCID
PMC322151
Subset
IM
Databases
GENBANK
X03242, X03243, X03244
External Links
PubMed source DOI: 10.1093/nar/13.23.8543 PMC Full Text View journal details
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