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PMID: 2997236 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Nerve growth factor-induced neurite outgrowth in PC12 cells involves the coordinate induction of microtubule assembly and assembly-promoting factors.

The Journal of cell biology ·Vol. 101 ·No. 5 Pt 1 ·1985-11-00 ·Pages 1799-807

Drubin DG, Feinstein SC, Shooter EM, Kirschner MW

Abstract

Nerve growth factor (NGF) regulates the microtubule-dependent extension and maintenance of axons by some peripheral neurons. We show here that one effect of NGF is to promote microtubule assembly during neurite outgrowth in PC12 cells. Though NGF causes an increase in total tubulin levels, the formation of neurites and the assembly of microtubules follow a time course completely distinct from that of the tubulin induction. The increases in microtubule mass and neurite extension closely parallel 10- and 20-fold inductions of tau and MAP1, proteins shown previously to promote microtubule assembly in vitro. When NGF is removed from PC12 cells, neurites disappear, microtubule mass decreases, and both microtubule-associated proteins return to undifferentiated levels. These data suggest that the induction of tau and MAP1 in response to NGF promotes microtubule assembly and that these factors are therefore key regulators of neurite outgrowth.

MeSH Terms
Adrenal Gland Neoplasms/ultrastructure Animals Bucladesine/pharmacology Cell Line Kinetics Mice Microtubule Proteins/metabolism Microtubule-Associated Proteins/biosynthesis Microtubules/drug effects,metabolism,ultrastructure Nerve Growth Factors/pharmacology Pheochromocytoma/ultrastructure Time Factors tau Proteins
Chemicals
Microtubule Proteins Microtubule-Associated Proteins Nerve Growth Factors tau Proteins Bucladesine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Drubin D G
Feinstein S C
Shooter E M
Kirschner M W
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43 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.101.5.1799
Published
1985-11-00
Pages
1799-807
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2113947
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.101.5.1799 PMC Full Text View journal details
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